Immunohistochemical localization of HCA2 receptors in different parts of rat gut

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

shabani pouria; hamedi somayeh

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN Year:2019 | Volume:16 | Issue:3 (66) Page(s): 2893-2900

Download Full-Text

Persian Verion

View:

293

Download:

Cites:

Information Journal Paper

Title

Immunohistochemical localization of HCA2 receptors in different parts of rat gut

Author(s)

shabani pouria | hamedi somayeh | Issue Writer Certificate

Keywords

GutQ2

RatQ2

HCA2 receptorQ1

ImmunohistochemistryQ2

Abstract

The hydroxy-carboxylic acid receptor 2 (HCA2 receptor) is the target of niacin. In the present study we describe the expression pattern of these receptors in cells of different parts of alimentary canal (from esophagus to rectum) of Rats by immunohistochemical method. Six adult male Wistar Rats were euthanized under deep anesthesia. Five μ m-paraffin transverse sections were made from esophagus, stomach duodenum, jejunum, ileum, cecum, colons and rectum. The primary antibody for immunohistochemical staining was rabbit polyclonal GPR109A antibody (1: 300, incubation for one night at 4° C) which reacts with Rat HCA2 receptor. Secondary antibody was HRP-conjugated. We observed weak immunoreactivity in epithelial cells of esophagus and non-glandular stomach, strong immunoreactivity of HCA2 receptors in epithelial cells of duodenum, jejunum, ileum and jejunal muscular layer. Moreover, colon showed weak and rectum showed no immunoreactivity. Immunoreactivity was strong in base of caecum. In conclusion, HCA2 receptor proteins are abundantly present in intestinal epithelium and base of caecum of Rats which make them a proper laboRatory animal for future studies on these receptors in gastrointestinal tract.

Cites

No record.

References

No record.

Cite

APA: Copy

shabani, pouria, & hamedi, somayeh. (2019). Immunohistochemical localization of HCA2 receptors in different parts of rat gut. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 16(3 (66) ), 2893-2900. SID. https://sid.ir/paper/375765/en

Vancouver: Copy

shabani pouria, hamedi somayeh. Immunohistochemical localization of HCA2 receptors in different parts of rat gut. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN[Internet]. 2019;16(3 (66) ):2893-2900. Available from: https://sid.ir/paper/375765/en

IEEE: Copy

pouria shabani, and somayeh hamedi, “Immunohistochemical localization of HCA2 receptors in different parts of rat gut,” JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, vol. 16, no. 3 (66) , pp. 2893–2900, 2019, [Online]. Available: https://sid.ir/paper/375765/en

Related Journal Papers