Anatical Review: Evaluation and Comparison of New Methods in HLA Typing

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GHAFARI N.; Raesi M.; GUITY K.; DANESHPOUR M.

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Paper Information

Journal: Pathobiology Research Year:2020 | Volume:23 | Issue:2 Page(s): 74-84

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Information Journal Paper

Title

Anatical Review: Evaluation and Comparison of New Methods in HLA Typing

Author(s)

GHAFARI N. | Raesi M. | GUITY K. | DANESHPOUR M. | Issue Writer Certificate

Keywords

Major Histocamplimentary System (MHC)Q1

Human Leukocyte Antigen (HLA)Q1

Next Generation of SequencingQ1

Ilumina MiseqQ1

Thermal Sequence DetectionQ1

Abstract

The human leukocyte antigen (HLA) system has unique genetic and biochemical complexes, so it is important to determine the type of HLA in a clinical and laboratory process. In addition, to its application to assess the risk of various diseases, it also plays a vital role in organ transplants. The determination of HLA in patients requiring transplantation, in addition to the accuracy, also requires the speed of action. So that any delay and error in this work can endanger the patient’ s life. The need for a precise definition of the HLA type, along with improved speed and cost reduction, has led to the introduction of several methods for this test. Today, the introduction of a new generation of revolutionary sequencing has been carried out in genetic assessments. The HLA determination also uses this technique, and a variety of methods have been introduced for genetic HLA examination using a new generation of sequencing. In the present study, along with the review of traditional methods in HLA diagnosis, two of the most widely used techniques based on a new generation of sequencing methods, Thermal Sequencing method and Illumina Miseq method were evaluated.

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References

Performance of Illumina Next Generation Sequencing

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APA: Copy

GHAFARI, N., Raesi, M., GUITY, K., & DANESHPOUR, M.. (2020). Anatical Review: Evaluation and Comparison of New Methods in HLA Typing. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 23(2 ), 74-84. SID. https://sid.ir/paper/376671/en

Vancouver: Copy

GHAFARI N., Raesi M., GUITY K., DANESHPOUR M.. Anatical Review: Evaluation and Comparison of New Methods in HLA Typing. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2020;23(2 ):74-84. Available from: https://sid.ir/paper/376671/en

IEEE: Copy

N. GHAFARI, M. Raesi, K. GUITY, and M. DANESHPOUR, “Anatical Review: Evaluation and Comparison of New Methods in HLA Typing,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 23, no. 2 , pp. 74–84, 2020, [Online]. Available: https://sid.ir/paper/376671/en

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