ISOLATION AND PURIFICATION OF RAT ISLET CELLS

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AKBARZADEH A.; JAMSHIDI SHIRIN; FARHANGI A.; LAMERAD B.; BAHREMAND S.

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Journal: Journal of Sabzevar University of Medical Sciences Year:2005 | Volume:12 | Issue:2 Page(s): 6-13

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Title

ISOLATION AND PURIFICATION OF RAT ISLET CELLS

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Keywords

FLOW CYTOMETRYQ3

PURIFICATIONQ3

ISOLATIONQ2

ISLET CELLSQ4

Abstract

Background and Purpose: It is thought that transplantation of ISLET CELLS would cure diabetic patients in world. ISLET CELLS transplantation in people suffering from diabetes is of technical interest. A standardized procedure was developed for the preparation of rat islet cell grafts for PURIFICATION of ISLET CELLS.Materials and methods: In this process, after collagens digestion of pancreases, islets were isolated and dissociated, then with enzymatic procedure by DNase and trypsin, the ISLET CELLS changed in to single cells and these cells were assayed by flow cytometery. Results: Flow cytometery of these cells indicated that there were 91% of beta cells in cell suspension. Most of the exocrine particles were lost during digestion.Conclusion: Purified endocrine islet cell grafts were prepared by pure beta-cells, with or without endocrine non-beta cells. The purified aggregates were devoid of non-endocrine cells and damaged cells.

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APA: Copy

AKBARZADEH, A., JAMSHIDI, SHIRIN, FARHANGI, A., LAMERAD, B., & BAHREMAND, S.. (2005). ISOLATION AND PURIFICATION OF RAT ISLET CELLS. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, 12(2), 6-13. SID. https://sid.ir/paper/378212/en

Vancouver: Copy

AKBARZADEH A., JAMSHIDI SHIRIN, FARHANGI A., LAMERAD B., BAHREMAND S.. ISOLATION AND PURIFICATION OF RAT ISLET CELLS. JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2005;12(2):6-13. Available from: https://sid.ir/paper/378212/en

IEEE: Copy

A. AKBARZADEH, SHIRIN JAMSHIDI, A. FARHANGI, B. LAMERAD, and S. BAHREMAND, “ISOLATION AND PURIFICATION OF RAT ISLET CELLS,” JOURNAL OF SABZEVAR UNIVERSITY OF MEDICAL SCIENCES, vol. 12, no. 2, pp. 6–13, 2005, [Online]. Available: https://sid.ir/paper/378212/en

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