Molecular identification of Strawberry mottle virus in strawberry fields of Kurdistan Province based on the 3΄ NCR

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Ghaderi Zandan N.; HAJIZADEH M.

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Journal: Applied Entomology and Phytopathology Year:2019 | Volume:87 | Issue:1 (108) Page(s): 107-116

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Title

Molecular identification of Strawberry mottle virus in strawberry fields of Kurdistan Province based on the 3΄ NCR

Author(s)

Ghaderi Zandan N. | HAJIZADEH M. | Issue Writer Certificate

Keywords

Genetic distanceQ1

phylogenyQ1

strawberryQ1

viral infectioQ1

Abstract

In the summer of 2018, viral symptoms corresponded to strawberry mottle virus (SMoV) in some strawberry fields were observed and 48 leaf samples/plants were collected from different fields from Kurdistan Province. In the preliminary test, some samples were inoculated to Fragaria vesca by strawberry aphid (Chaetosiphon fragaefolii) and SMoV-symptoms were observed after about two weeks. Then, total RNA of samples was extracted by silica-capture method and subjected to cDNA synthesize with random hexamer primers. RT-PCR was done by specific SMoV pair primer and amplified fragments were observed on 1. 2% agarose gels. RT-PCR results were showed that 28 out of 48 samples were infected by SMoV. Based on the geographical origin, cultivar and symptoms, five isolates were selected, ligated to pTG-19 and transformed to Escherichia coli. Recombinant plasmids were sequenced and analyzed for molecular characterization and phylogenetic studies on SMoV 3΄ NCR. Mean Genetic distance between the isolates from Iran was 0. 005 ± 0. 002 but between these isolates and the other isolates available in GenBank was 0. 084 ± 0. 008. The highest and lowest Genetic distance was found between K3 with NSper51 (0. 093 ± 0. 008) and SQA/AG with NB926 (0. 019 ± 0. 017) from Canada, respectively. This is the first report on occurrence of SMoV on strawberry in Kurdistan Province.

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APA: Copy

Ghaderi Zandan, N., & HAJIZADEH, M.. (2019). Molecular identification of Strawberry mottle virus in strawberry fields of Kurdistan Province based on the 3΄ NCR. APPLIED ENTOMOLOGY AND PHYTOPATHOLOGY, 87(1 (108) ), 107-116. SID. https://sid.ir/paper/379187/en

Vancouver: Copy

Ghaderi Zandan N., HAJIZADEH M.. Molecular identification of Strawberry mottle virus in strawberry fields of Kurdistan Province based on the 3΄ NCR. APPLIED ENTOMOLOGY AND PHYTOPATHOLOGY[Internet]. 2019;87(1 (108) ):107-116. Available from: https://sid.ir/paper/379187/en

IEEE: Copy

N. Ghaderi Zandan, and M. HAJIZADEH, “Molecular identification of Strawberry mottle virus in strawberry fields of Kurdistan Province based on the 3΄ NCR,” APPLIED ENTOMOLOGY AND PHYTOPATHOLOGY, vol. 87, no. 1 (108) , pp. 107–116, 2019, [Online]. Available: https://sid.ir/paper/379187/en

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