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Information Journal Paper

Title

Antibacterial effects Pulicaria gnaphalodes extract on some gram positive and negative bacteria

Pages

  516-524

Abstract

 Background: The general tendency of the community to use herbal medicines is due to prove the destructive effects of chemical drugs and environmental pollution. This study aimed to investigate the Anti-bacterial properties of P. gnaphalodes extract on some gram-positive and negative bacteria. Materials and Methods: In this study, all of the organs of the P. gnaphalodes plant was dried in the shade in the summer of 2016 after collection. The Anti-bacterial effect of the plant was measured on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus to obtain the best extraction method. Next, 15 ml of Muller-Hinton-Agar medium was mixed with concentrations of 10-5. 2-0. 25-0. 625 and 20 mg/ml of the extracts and 104 CFU /ml of the bacterium was inoculated and cultured after growing the colonies. In order to determine the MIC and MBC, the same volumes of 105 CFU/ ml were inoculated into Muller Hinton broth medium containing different concentrations of herbicide extracts. The culture medium was incubated for 24 hours at 37 ° C. Results: Concentration of 20 mg/ml had the most effect on inhibition of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus bacteria, which was eliminated for 90, 68, 75 and 90% bacteria, respectively. MIC and MBC of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus were obtained at 25, 50, 50, 70, 50, 80 and 30 and 50 mg/ml, respectively. Conclusion: The results showed that the extract of P. gnaphalodes has anti-bacterial effects on some gram positive and negative bacteria.

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    APA: Copy

    Jamshidi Moghaddam, y., RAJABIZADEH, A., & KHAZAELI, P.. (2020). Antibacterial effects Pulicaria gnaphalodes extract on some gram positive and negative bacteria. FEYZ, 24(5 ), 516-524. SID. https://sid.ir/paper/379316/en

    Vancouver: Copy

    Jamshidi Moghaddam y., RAJABIZADEH A., KHAZAELI P.. Antibacterial effects Pulicaria gnaphalodes extract on some gram positive and negative bacteria. FEYZ[Internet]. 2020;24(5 ):516-524. Available from: https://sid.ir/paper/379316/en

    IEEE: Copy

    y. Jamshidi Moghaddam, A. RAJABIZADEH, and P. KHAZAELI, “Antibacterial effects Pulicaria gnaphalodes extract on some gram positive and negative bacteria,” FEYZ, vol. 24, no. 5 , pp. 516–524, 2020, [Online]. Available: https://sid.ir/paper/379316/en

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