Staphylococcal Cassette Chromosome mec (SCCmec) Typing of Clinical Isolates of staphylococcus aureus by Multiplex PCR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Zerehsaz Javad; NAJAR PEERAYEH SHAHIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2019 | Volume:24 | Issue:85 Page(s): 33-40

Download Full-Text

Persian Verion

View:

385

Download:

Cites:

Information Journal Paper

Title

Staphylococcal Cassette Chromosome mec (SCCmec) Typing of Clinical Isolates of staphylococcus aureus by Multiplex PCR

Author(s)

Zerehsaz Javad | NAJAR PEERAYEH SHAHIN | Issue Writer Certificate

Keywords

Staphylococcus aureusQ2

MRSAQ2

multiplex PCRQ1

SCCmecQ2

Abstract

Background and objective: The widespread use of various antibiotics has been responsible for the resistance of Staphylococcus aureus to the antibiotics. Methicillin resistance has become especially important because of limiting therapeutic options. Therefore, in this study we evaluated drug resistance and genotyping of staphylococcal cassette chromosome mec (SCCmec) in S. aureus strains isolated from patients admitted in Tehran hospitals. Materials and methods: The 215 clinical isolates of S. aureus was collected from clinical specimens. Antibiotic susceptibility was determined by disk diffusion. PCR was performed to identify the mecA gene. Different types of SCC mec were identified using the multiplex PCR method. Result: The highest resistance was observed for tetracyclin (49. 3%), oxacilin (46. 04%) and erythromcin (36. 5%), followed by ciprofloxacin (29. 7%), gentamycin (28. 3%), clindamycin (27. 4%), co-trimoxazole (23. 3%) and vancomycin (0). The frequency of mecA gene was 46. 44%. multiplex PCR analysis showed that the distribution of different types of SCCmec in this study was the SCCmecIII (88. 9%), SCCmecII (03. 03%), SCCmecI (02. 2%), and none typable (6. 5%). Conclusion: It seems that there is a genetic diversity among MRSA circulating in studied hospitals that highlights the need to implement appropriate infection control policies in order to decrease dissemination of multi-drug resistance MRSA types in our hospitals.

Cites

No record.

References

No record.

Cite

APA: Copy

Zerehsaz, Javad, & NAJAR PEERAYEH, SHAHIN. (2019). Staphylococcal Cassette Chromosome mec (SCCmec) Typing of Clinical Isolates of staphylococcus aureus by Multiplex PCR. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 24(85 ), 33-40. SID. https://sid.ir/paper/381674/en

Vancouver: Copy

Zerehsaz Javad, NAJAR PEERAYEH SHAHIN. Staphylococcal Cassette Chromosome mec (SCCmec) Typing of Clinical Isolates of staphylococcus aureus by Multiplex PCR. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2019;24(85 ):33-40. Available from: https://sid.ir/paper/381674/en

IEEE: Copy

Javad Zerehsaz, and SHAHIN NAJAR PEERAYEH, “Staphylococcal Cassette Chromosome mec (SCCmec) Typing of Clinical Isolates of staphylococcus aureus by Multiplex PCR,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 24, no. 85 , pp. 33–40, 2019, [Online]. Available: https://sid.ir/paper/381674/en

Related Journal Papers