DETERMINATION OF E-CADHERIN AND VIMENTIN GENES EXPRESSION ON THE TUMOR SPECIMENS OF OVARIAN CANCER PATIENTS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

REZAZADEH SEYEDEH HAKIMEH; SHIRKOOHI REZA; ANGAJI ABDOLHAMID; SEYEDENA SEYED YUSEF; EMAMI RAZAVI AMIR NADER

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2018 | Volume:76 | Issue:2 Page(s): 111-119

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

4,329

Download:

Cites:

Information Journal Paper

Title

DETERMINATION OF E-CADHERIN AND VIMENTIN GENES EXPRESSION ON THE TUMOR SPECIMENS OF OVARIAN CANCER PATIENTS

Author(s)

Keywords

EPITHELIAL-MESENCHYMAL TRANSITIONQ1

E-CADHERINQ1

OVARIAN EPITHELIAL CANCERQ1

POLYMERASE CHAIN REACTIONQ1

VIMENTINQ3

Abstract

Background: Ovarian cancer is a leading metastatic disease. The epithelial ovarian cancer is one of the most common malignant cancers that usually remains asymptomatic up to metastasis stages, and most patient when diagnosed are in the advanced stage of the disease. Studies have shown that in the majority of epithelial cancers mesenchymal factor expression such as VIMENTIN increases, and the epithelial factor expression such as E-CADHERIN decreases, as a result, it causes an epithelialmesenchymal transition (EMT). The aim of this study was to determine the expression level of these genes and association between EMT phenomenon and development of ovarian cancer based on clinical and morphological findings.Methods: In the present case series study, 70 samples were chosen from the tumor Bank of Cancer Institute taken from patients at Imam Khomeini Hospital, Tehran, Iran.The amount of expression of two genes, E-CADHERIN and VIMENTIN, was investigated by real-time PCR method from February 2016 to September 2017. The RNA extraction was done manually, and then cDNA synthesis was performed; In each sample the expression level of VIMENTIN and E-CADHERIN was measured with real-time PCR method.The patient’s clinical information with other data were analyzed with nonparametric statistical methods in SPSS software, version 19 (SPSS Inc., Chicago, IL, USA).Results: There was a significant relationship between expression of VIMENTIN gene and the stage (P=0.026) of the disease and metastasis (P=0.009), There was no significant relationship between VIMENTIN gene expression and tumor grade (P=0.207), age (P=0.11), tumor size (P=0.71) and family history (P=0.6). There was a significant correlation between E-CADHERIN gene expression and metastasis (P=0.027), no significant correlation was found between E-CADHERIN gene expression with tumor grade (P=0.690), stage (P=0.753), age (P=0.09), tumor size (P=0.537) and family history (P=0.56).Conclusion: According to the changes in expression of VIMENTIN and E-CADHERIN genes in ovarian tumor cells, and association between these two genes with clinical and morphological findings and the role of these genes in the migration and invasion, we can use the both genes, VIMENTIN and E-CADHERIN, as genes involved in the EMT process to assess disease progression and incidence of cell invasion in ovarian cancer.

Cites

No record.

References

No record.

Cite

APA: Copy

REZAZADEH, SEYEDEH HAKIMEH, SHIRKOOHI, REZA, ANGAJI, ABDOLHAMID, SEYEDENA, SEYED YUSEF, & EMAMI RAZAVI, AMIR NADER. (2018). DETERMINATION OF E-CADHERIN AND VIMENTIN GENES EXPRESSION ON THE TUMOR SPECIMENS OF OVARIAN CANCER PATIENTS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 76(2 ), 111-119. SID. https://sid.ir/paper/38462/en

Vancouver: Copy

REZAZADEH SEYEDEH HAKIMEH, SHIRKOOHI REZA, ANGAJI ABDOLHAMID, SEYEDENA SEYED YUSEF, EMAMI RAZAVI AMIR NADER. DETERMINATION OF E-CADHERIN AND VIMENTIN GENES EXPRESSION ON THE TUMOR SPECIMENS OF OVARIAN CANCER PATIENTS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2018;76(2 ):111-119. Available from: https://sid.ir/paper/38462/en

IEEE: Copy

SEYEDEH HAKIMEH REZAZADEH, REZA SHIRKOOHI, ABDOLHAMID ANGAJI, SEYED YUSEF SEYEDENA, and AMIR NADER EMAMI RAZAVI, “DETERMINATION OF E-CADHERIN AND VIMENTIN GENES EXPRESSION ON THE TUMOR SPECIMENS OF OVARIAN CANCER PATIENTS,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 76, no. 2 , pp. 111–119, 2018, [Online]. Available: https://sid.ir/paper/38462/en

Related Journal Papers