COMPARISON OF AGGRECAN GENE EXPRESSION IN CHONDROGENESIS OF ADIPOSEDERIVED STEM CELLS IN PELLET AND MICROMASS CULTURE SYSTEMS

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TEIMOURI MAHTAB; HASHEMIBENI BATOOL; MARDANI MOHAMMAD

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Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2018 | Volume:76 | Issue:2 Page(s): 90-95

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Title

COMPARISON OF AGGRECAN GENE EXPRESSION IN CHONDROGENESIS OF ADIPOSEDERIVED STEM CELLS IN PELLET AND MICROMASS CULTURE SYSTEMS

Author(s)

TEIMOURI MAHTAB | HASHEMIBENI BATOOL | MARDANI MOHAMMAD | Issue Writer Certificate

Keywords

CHONDROGENESISQ2

AGGRECANQ2

MICROMASS CULTUREQ1

PELLETQ1

Abstract

Background: Nowadays, Human adipocyte-derived stem cells (hADSCs) has been widely used in tissue engineering because of its unique features such as extraction from more sources, more easily and non-invasive extraction methods. In order to increase cell-cell interactions, similar to embryonic pre-cartilage condensation, the use of three dimensional (3D) high-density cell culture systems such as PELLET and Micromass that simulates optimal condensation in CHONDROGENESIS in vivo is necessary. Also, these culture systems provide the proper diffusion of nutrients. AGGRECAN is a proteoglycan and one of the important components of extracellular matrix of cartilage tissue that plays an important role in the organization of the extracellular matrix. The high concentrations of AGGRECAN produces the osmotic properties that is necessary to normal tissue function of cartilage. In current study, AGGRECAN gene expression wasinvestigated during CHONDROGENESIS of hADSCs in two PELLET and MICROMASS CULTURE systems.Methods: This experimental study was done in Department of Anatomical Sciences Department of Faculty Medical in Isfahan University of Medical Sciences, Iran, from April 2013 to January 2015. First, the abdominal adipose tissue was obtained from three patients after obtaining written consent during their liposuction surgeries. ADSCs were extracted by mechanical and enzymatic methods and were cultured in monolayer culture. Then, in order to induction of chondrogenic differentiation, 5×105 cells of third passage (P3) were transferred to three-dimensional culture systems PELLET and Micromass containing chondrogenic mediums in experimental groups of 7 and 14 days.The evaluation of AGGRECAN gene expression was performed by real-time PCR technique.Results: Gene expression analysis revealed that AGGRECAN was significantly increased in MICROMASS CULTURE at day 14 compared to PELLET culture at days 14 and 7 (P£0.01). Also, AGGRECAN was significantly increased in MICROMASS CULTURE at day 7 compared to PELLET culture at day 7 (P£0.05).Conclusion: Due to higher expression of AGGRECAN gene in MICROMASS CULTURE compared to PELLET culture, this system may be more efficient than PELLET culture in synthesis of AGGRECAN in chondrogenic differentiation of ADSCs.

Cites

Adipose-Derived Stem Cells Conditioned Media Promote In Vitro Osteogenic Differentiation of Hypothyroid Mesenchymal Stem Cells

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APA: Copy

TEIMOURI, MAHTAB, HASHEMIBENI, BATOOL, & MARDANI, MOHAMMAD. (2018). COMPARISON OF AGGRECAN GENE EXPRESSION IN CHONDROGENESIS OF ADIPOSEDERIVED STEM CELLS IN PELLET AND MICROMASS CULTURE SYSTEMS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 76(2 ), 90-95. SID. https://sid.ir/paper/38464/en

Vancouver: Copy

TEIMOURI MAHTAB, HASHEMIBENI BATOOL, MARDANI MOHAMMAD. COMPARISON OF AGGRECAN GENE EXPRESSION IN CHONDROGENESIS OF ADIPOSEDERIVED STEM CELLS IN PELLET AND MICROMASS CULTURE SYSTEMS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2018;76(2 ):90-95. Available from: https://sid.ir/paper/38464/en

IEEE: Copy

MAHTAB TEIMOURI, BATOOL HASHEMIBENI, and MOHAMMAD MARDANI, “COMPARISON OF AGGRECAN GENE EXPRESSION IN CHONDROGENESIS OF ADIPOSEDERIVED STEM CELLS IN PELLET AND MICROMASS CULTURE SYSTEMS,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 76, no. 2 , pp. 90–95, 2018, [Online]. Available: https://sid.ir/paper/38464/en

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