Osteoblastic Differentiation of Amniotic Pluripotent Stem Cell

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MAHMOODINIA MAYMAND M.; NORUZINIA M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: SAREM JOURNAL OF MEDICAL RESEARCH (SAREM JOURNAL OF REPRODUCTIVE MEDICINE) Year:2017 | Volume:1 | Issue:1 Page(s): 9-13

Download Full-Text

Persian Verion

View:

386

Download:

Cites:

Information Journal Paper

Title

Osteoblastic Differentiation of Amniotic Pluripotent Stem Cell

Author(s)

MAHMOODINIA MAYMAND M. | NORUZINIA M. | Issue Writer Certificate

Keywords

Fetal Stem Cells

Amniotic Fluid

Osteoblasts

Cell Differentiation

Abstract

Aims Amniotic Fluid stem cells have lower ethical limitations than embryonic stem cells for the use in research and treatment. These cells show great self-renewal potential and can differentiate into the specialized cells of all three germ layers. The Amniotic Fluid stem cells display minimal risks of teratomas and very low immunogenicity. For these reasons, Amniotic Fluid appears as a promising alternative source for stem cell therapy. The objective of this study was to isolate the stem cells from Amniotic Fluid and differentiate them into the osteoblastic cells. Materials & Methods An Amniotic Fluid sample (about 10ml) was collected from a healthy donor in Sarem women’ s hospital (Tehran, Iran). After centrifugation, the cells were cultured in a DMEM medium supplemented with 20% FBS. The cell clones were observed after two weeks and were passaged to an osteoblastic differentiation medium. Alizarin red staining and RTPCR for alkaline phosphatase and osteocalcin markers were used for confirmation of cellular differentiation. Findings Stem cells were isolated from Amniotic Fluid. Phenotypically, these cells showed spindle-shaped morphology with a large nucleus. Following the induction of differentiation, they showed the expression of osteoblastic cells markers indicating their differentiation. The expression of those markers was confirmed by immunocytochemistry and RT-PCR. Conclusion Amniotic stem cells have the ability to differentiate into the osteoblastic cells using an osteoblastic differentiation medium.

Cites

No record.

References

No record.

Cite

APA: Copy

MAHMOODINIA MAYMAND, M., & NORUZINIA, M.. (2017). Osteoblastic Differentiation of Amniotic Pluripotent Stem Cell. SAREM JOURNAL OF MEDICAL RESEARCH (SAREM JOURNAL OF REPRODUCTIVE MEDICINE), 1(1 ), 9-13. SID. https://sid.ir/paper/385139/en

Vancouver: Copy

MAHMOODINIA MAYMAND M., NORUZINIA M.. Osteoblastic Differentiation of Amniotic Pluripotent Stem Cell. SAREM JOURNAL OF MEDICAL RESEARCH (SAREM JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2017;1(1 ):9-13. Available from: https://sid.ir/paper/385139/en

IEEE: Copy

M. MAHMOODINIA MAYMAND, and M. NORUZINIA, “Osteoblastic Differentiation of Amniotic Pluripotent Stem Cell,” SAREM JOURNAL OF MEDICAL RESEARCH (SAREM JOURNAL OF REPRODUCTIVE MEDICINE), vol. 1, no. 1 , pp. 9–13, 2017, [Online]. Available: https://sid.ir/paper/385139/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops