Effect of different concentrations of melatonin on SSCs colony formation (In vitro)

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Jamshidi-Seykevandi Hesam; RAHIMI FEYLI PEYMAN; MOGHADDAM ALIASGHAR

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Journal: Research in Medicine Year:2021 | Volume:44 | Issue:4 Page(s): 580-586

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Title

Effect of different concentrations of melatonin on SSCs colony formation (In vitro)

Author(s)

Jamshidi-Seykevandi Hesam | RAHIMI FEYLI PEYMAN | MOGHADDAM ALIASGHAR | Issue Writer Certificate

Keywords

Spermatogonial Stem cellQ1

Colony formationQ1

MelatoninQ2

Abstract

Background: Spermatogonial Stem cells (SSCs) are used as an excellent model system for differentiation, development, and functioning of testes and provide a source of cells for infertility treatment and production of transgenic animals and recombinant drugs. The aim of the present study was to determine the effects of different concentrations of Melatonin on prepubertal lamb's spermatogonial Colony formation. Methods: An experimental study was conducted. Each treatment was replicated 5 times. Testicular cells were isolated from testes of prepubertal lambs using two-step enzymatic digestion and then cultured for 10 days. Control cells (C) were grown in basic DMEM media with %1 antibiotic and %5 FBS. Treated cells were grown in basic media with 1 µ mol/ml (T1) or 1 nmol/ml (T2) Melatonin. Culture media was changed every 72h and SSCs Colony formation was monitored via inverted microscope. Data were analyzed running one way ANOVA. P values less than %5 were considered as statistically significant. Results: SSCs were identified via immunocytochemistry assay against PGP9. 5. Number and surface area of colonies were greater in T1 group than C and T2 groups on day 7 (P=0. 04587). Conclusion: The findings suggest that simultaneous addition of 1 µ mol/ml Melatonin to culture media may increase in vitro Colony formation of prepubertal sheep SSCs. So, addition of above dose of Melatonin to SSCs culture media is suggested.

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APA: Copy

Jamshidi-Seykevandi, Hesam, RAHIMI FEYLI, PEYMAN, & MOGHADDAM, ALIASGHAR. (2021). Effect of different concentrations of melatonin on SSCs colony formation (In vitro). PAJOUHESH DAR PEZESHKI, 44(4 ), 580-586. SID. https://sid.ir/paper/385676/en

Vancouver: Copy

Jamshidi-Seykevandi Hesam, RAHIMI FEYLI PEYMAN, MOGHADDAM ALIASGHAR. Effect of different concentrations of melatonin on SSCs colony formation (In vitro). PAJOUHESH DAR PEZESHKI[Internet]. 2021;44(4 ):580-586. Available from: https://sid.ir/paper/385676/en

IEEE: Copy

Hesam Jamshidi-Seykevandi, PEYMAN RAHIMI FEYLI, and ALIASGHAR MOGHADDAM, “Effect of different concentrations of melatonin on SSCs colony formation (In vitro),” PAJOUHESH DAR PEZESHKI, vol. 44, no. 4 , pp. 580–586, 2021, [Online]. Available: https://sid.ir/paper/385676/en

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