Drought stress tolerance mechanism and expression of genes involved in osmotic regulation in some wheat and wild crop species

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KEIKHA M.; Mahdi Nezhad n.; FAKHERI B.; MOHAMADI R.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2019 | Volume:14 | Issue:2 Page(s): 111-123

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Title

Drought stress tolerance mechanism and expression of genes involved in osmotic regulation in some wheat and wild crop species

Author(s)

KEIKHA M. | Mahdi Nezhad n. | FAKHERI B. | MOHAMADI R. | Issue Writer Certificate

Keywords

Gene expressionQ2

ProlineQ1

GlycinebetaineQ1

Real Time PCRQ1

Abstract

In order to study the mechanism of drought tolerance and expression of the genes involved in osmotic regulation in wheat, a factorial experiment based on a randomized design with three replication, in two condition of non-stress and without drought stress were carried out. Three Diploid species, two kind of Tetraplepoloid and two kind of Hexaploid in the greenhouse of university Zabol, were examined in a pot. The evaluated physiological traits were included Proline, Glycine betaine, total Soluble Carbohydrate, total Protein, RWC (relative humidity). Also, the pattern of gene relative expression of Prolin (P5CS) and Glycinebetaine (BADH) expression was measured using Real Time PCR. Drought stress increased total Protein, total Soluble Carbohydrate, Proline, Glycinebetaine and significantly reduced relative water content, and drought stress induced expression of P5CS and BADH in all cultivars. Therefore, osmotic regulation and expression of two P5CS and BADH expressions under stress conditions can be used as an indicator in breeding programs to select drought tolerant cultivars.

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APA: Copy

KEIKHA, M., Mahdi Nezhad, n., FAKHERI, B., & MOHAMADI, R.. (2019). Drought stress tolerance mechanism and expression of genes involved in osmotic regulation in some wheat and wild crop species. MODERN GENETICS JOURNAL (MGJ), 14(2 ), 111-123. SID. https://sid.ir/paper/386523/en

Vancouver: Copy

KEIKHA M., Mahdi Nezhad n., FAKHERI B., MOHAMADI R.. Drought stress tolerance mechanism and expression of genes involved in osmotic regulation in some wheat and wild crop species. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2019;14(2 ):111-123. Available from: https://sid.ir/paper/386523/en

IEEE: Copy

M. KEIKHA, n. Mahdi Nezhad, B. FAKHERI, and R. MOHAMADI, “Drought stress tolerance mechanism and expression of genes involved in osmotic regulation in some wheat and wild crop species,” MODERN GENETICS JOURNAL (MGJ), vol. 14, no. 2 , pp. 111–123, 2019, [Online]. Available: https://sid.ir/paper/386523/en

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