AN OPTIMIZED MIXTURE OF KIWIFRUIT ACTINIDIN AND TRYPSIN FOR ISOLATION AND CULTURE OF ENDOTHELIAL CELLS FROM RAT AORTA

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HESARI MAHVASH; MANSOURI KAMRAN; MOSTAFAIE ALI; BIDMESHKIPOUR ALI

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Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2010 | Volume:68 | Issue:3 Page(s): 153-160

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Title

AN OPTIMIZED MIXTURE OF KIWIFRUIT ACTINIDIN AND TRYPSIN FOR ISOLATION AND CULTURE OF ENDOTHELIAL CELLS FROM RAT AORTA

Author(s)

HESARI MAHVASH | MANSOURI KAMRAN | MOSTAFAIE ALI | BIDMESHKIPOUR ALI | Issue Writer Certificate

Keywords

ACTINIDINQ3

TRYPSINQ3

ENDOTHELIAL CELLSQ2

AORTAQ3

RATQ3

COLLAGENASEQ3

Abstract

Background: Proteolytic enzymes, especially COLLAGENASEs, are used for digestion of extracellular matrix, cell isolation and primary culture. Because of the problems in purification and low amount of COLLAGENASEs in bacterial or animal sources, it is important to find new sources of the enzymes. So, in the present study ACTINIDIN, a plentiful protein in kiwifruit was purified and a mixture of ACTINIDIN and TRYPSIN was applied to isolate RAT aortic ENDOTHELIAL CELLS.Methods: Aortic ENDOTHELIAL CELLS were isolated using digestion solution containing different concentrations of ACTINIDIN (from 2 to 16 mg/ml) and TRYPSIN (0.3, 0.6, 1.2 and 2.4 mg/ml) in different times (from 15 to 90 minute). Isolated cells were cultured in DMEM culture medium. Isolated cells were identified by morphological characteristics and immunocytochemical staining; viability of separated cells was estimated by trypan blue exclusion test.Results: ACTINIDIN in concentration of 10 mg/ml with TRYPSIN in concentration of 1.2 mg/ml for one hour could isolate RAT aortic ENDOTHELIAL CELLS. In this condition the viability of cells was estimated 90%. Morphological and immunocytochemical characteristics confirmed the isolated cells as ENDOTHELIAL CELLS.Conclusion: The results showed that the mentioned mixture of ACTINIDIN and TRYPSIN has not considerable toxic effects on separated cells and is a novel and suitable option for isolation of RAT aortic ENDOTHELIAL CELLS.

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APA: Copy

HESARI, MAHVASH, MANSOURI, KAMRAN, MOSTAFAIE, ALI, & BIDMESHKIPOUR, ALI. (2010). AN OPTIMIZED MIXTURE OF KIWIFRUIT ACTINIDIN AND TRYPSIN FOR ISOLATION AND CULTURE OF ENDOTHELIAL CELLS FROM RAT AORTA. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 68(3), 153-160. SID. https://sid.ir/paper/38703/en

Vancouver: Copy

HESARI MAHVASH, MANSOURI KAMRAN, MOSTAFAIE ALI, BIDMESHKIPOUR ALI. AN OPTIMIZED MIXTURE OF KIWIFRUIT ACTINIDIN AND TRYPSIN FOR ISOLATION AND CULTURE OF ENDOTHELIAL CELLS FROM RAT AORTA. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2010;68(3):153-160. Available from: https://sid.ir/paper/38703/en

IEEE: Copy

MAHVASH HESARI, KAMRAN MANSOURI, ALI MOSTAFAIE, and ALI BIDMESHKIPOUR, “AN OPTIMIZED MIXTURE OF KIWIFRUIT ACTINIDIN AND TRYPSIN FOR ISOLATION AND CULTURE OF ENDOTHELIAL CELLS FROM RAT AORTA,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 68, no. 3, pp. 153–160, 2010, [Online]. Available: https://sid.ir/paper/38703/en

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