THE EFFECT OF WET CUPPING ON SERUM LEVELS OF INTERFERON-GAMMA IN LABORATORY RATS WITH THE DISEASE BRUCELLOSIS

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MOLAVI MOHAMMAD; YOUSEFI MASHOUF RASOUL; ALIKHANI MOHAMMAD YOUSEF; MAHMOUDI HASSAN; ZAMANI ALIREZA; KERAMAT FARIBA; SOLEIMANI ASL SARA

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Journal: Tehran University Medical Journal Year:2017 | Volume:75 | Issue:6 Page(s): 449-456

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Title

THE EFFECT OF WET CUPPING ON SERUM LEVELS OF INTERFERON-GAMMA IN LABORATORY RATS WITH THE DISEASE BRUCELLOSIS

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Keywords

BRUCELLOSISQ3

CONTROL GROUPSQ1

INTERFERON-GAMMAQ1

MERCAPTOETHANOLQ2

RATSQ3

Abstract

Background: BRUCELLOSIS is a systemic infection caused by gram-negative coccobacilli and facultative intracellular bacteria of the genus brucella. BRUCELLOSIS is a bacterial disease common to humans and livestock. Infection with Brucella spp. continues to pose a human health risk globally despite strides in eradicating the disease from domestic animals. The humoral and cellular immunity plays an important role in BRUCELLOSIS. The effect of phagocytosis and cellular immunity has been demonstrated in BRUCELLOSIS. The effect of cupping as a therapeutic method on bacterial diseases has been demonstrated. By considering this fact that cupping is an effective method on host immune system functions and has potential effect to regulate the inflammatory reactions.Methods: This experimental study was carried out on 48 RATS in 6 groups (48 RATS were divided into 6 groups) during the first 6 months from January 2015 to July 2016 in the laboratory of microbiology and animal of Hamedan University of Medical Sciences.The RATS were infected through the injection ofBrucella melitensis with plenty 5×105 (cfu/ml) of bacteria. After a week, in order to approve the accuracy of the infection in the studied RATS inoculated with coombosBrucella melitensis, Wright test, 2-Mercaptoethanol test and Coombs' Wright test were used. The RATS were then treated with cupping method in their sacral area. IFN-gwas measured using enzyme-linked immunosorbent assay. And the study of tissues using hematoxylin and eosin (H& E) Staining.Results: The results of this study showed that cupping leads to an increase in the mean serum level of INTERFERON-GAMMA. The histopathological study of liver tissue showed that the radial arrangement was not observed in the infected group with BRUCELLOSIS and the cells were acidophilus. While, the radial arrangement was observed in treated RATS with cupping, but it was not complete. The number of enlarged sinusoids were reduced.But, cell infiltration was observed.Conclusion: This study showed that cupping can increase serum level of IFN-g, and thus help to the clearance of disease and improvement of injury in the BRUCELLOSIS animals lab.

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APA: Copy

MOLAVI, MOHAMMAD, YOUSEFI MASHOUF, RASOUL, ALIKHANI, MOHAMMAD YOUSEF, MAHMOUDI, HASSAN, ZAMANI, ALIREZA, KERAMAT, FARIBA, & SOLEIMANI ASL, SARA. (2017). THE EFFECT OF WET CUPPING ON SERUM LEVELS OF INTERFERON-GAMMA IN LABORATORY RATS WITH THE DISEASE BRUCELLOSIS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 75(6), 449-456. SID. https://sid.ir/paper/38729/en

Vancouver: Copy

MOLAVI MOHAMMAD, YOUSEFI MASHOUF RASOUL, ALIKHANI MOHAMMAD YOUSEF, MAHMOUDI HASSAN, ZAMANI ALIREZA, KERAMAT FARIBA, SOLEIMANI ASL SARA. THE EFFECT OF WET CUPPING ON SERUM LEVELS OF INTERFERON-GAMMA IN LABORATORY RATS WITH THE DISEASE BRUCELLOSIS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2017;75(6):449-456. Available from: https://sid.ir/paper/38729/en

IEEE: Copy

MOHAMMAD MOLAVI, RASOUL YOUSEFI MASHOUF, MOHAMMAD YOUSEF ALIKHANI, HASSAN MAHMOUDI, ALIREZA ZAMANI, FARIBA KERAMAT, and SARA SOLEIMANI ASL, “THE EFFECT OF WET CUPPING ON SERUM LEVELS OF INTERFERON-GAMMA IN LABORATORY RATS WITH THE DISEASE BRUCELLOSIS,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 75, no. 6, pp. 449–456, 2017, [Online]. Available: https://sid.ir/paper/38729/en

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