DETECTION OF CHLAMYDIA TRACHOMATIS AND NEISSERIA GONORRHOAE BY PCR AND
MULTIPLEX PCR FROM NON-INVASIVE GENITO-URINARY SPECIMEN OF PATIENTS WITH URETHRITIS

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FATH ELAHZADEH B.; MIRSALEHIAN A.; KAZEMI B.; ARSHADI H.; POURAKBARI B.

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Journal: Tehran University Medical Journal Year:2004 | Volume:62 | Issue:6 Page(s): 449-456

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Title

DETECTION OF CHLAMYDIA TRACHOMATIS AND NEISSERIA GONORRHOAE BY PCR AND MULTIPLEX PCR FROM NON-INVASIVE GENITO-URINARY SPECIMEN OF PATIENTS WITH URETHRITIS

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Keywords

CHLAMYDIA TRACHOMATISQ4

NEISSERIA GONORRHOEAEQ4

MULTIPLEX PCRQ4

URETHRITISQ4

FIRST VOID URINE (FVU)Q4

Abstract

Background: Genitourinary tract infections due to CHLAMYDIA TRACHOMATIS and Neisseria gonorrhoae are a major cause of morbidity in sexually active individuals. Therefore, availability of a rapid method with high sensitivity and specificity that is capable of detecting both organisms from non-invasive specimen is highly desirable. Materials and Methods: In this study 10 to 15 ml samples of first void urine (FVU) from sixty-seven (62 males and 5 females), afflicted patients with URETHRITIS were examined. In addition discharges from the beginning of urethral duct were collected for bacteriologic study. Each sample was cultured on special culture for N.gonorrhoeae. After extracting the DNA from these samples, the PCR and MULTIPLEX PCR reactions were done and amplicons were examined by Agar Gel Electrophoresis system.Results: The results of this study showed that thirty-one (46%) of the samples with N. gonorrhoeae and fifteen (22%) of the samples with C. trachomatis were positive. In seven (10%) cases, infection with both of bacteria were observed, while the growth of N. gonorrhoeae on special culture were observed only in twenty-five (37.3%) of the samples. The KAPA coefficient indicating the degree of agreement of MULTIPLEX PCR and uniplex PCRswas equal to one.Conclusion: MULTIPLEX PCR provides a rapid and simultaneous test for detection of CHLAMYDIA TRACHOMATIS and Neisseria gonorrhoae in first void urine specimens collected by non-invasive procedure. Testing of such samples by MULTIPLEX PCR could facilitate studies aimed at improving our understanding of the epidemiology of CHLAMYDIA TRACHOMATIS and Neisseria gonorrhoae as important sexually transmitted diseases.

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APA: Copy

FATH ELAHZADEH, B., MIRSALEHIAN, A., KAZEMI, B., ARSHADI, H., & POURAKBARI, B.. (2004). DETECTION OF CHLAMYDIA TRACHOMATIS AND NEISSERIA GONORRHOAE BY PCR AND MULTIPLEX PCR FROM NON-INVASIVE GENITO-URINARY SPECIMEN OF PATIENTS WITH URETHRITIS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 62(6), 449-456. SID. https://sid.ir/paper/38812/en

Vancouver: Copy

FATH ELAHZADEH B., MIRSALEHIAN A., KAZEMI B., ARSHADI H., POURAKBARI B.. DETECTION OF CHLAMYDIA TRACHOMATIS AND NEISSERIA GONORRHOAE BY PCR AND MULTIPLEX PCR FROM NON-INVASIVE GENITO-URINARY SPECIMEN OF PATIENTS WITH URETHRITIS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2004;62(6):449-456. Available from: https://sid.ir/paper/38812/en

IEEE: Copy

B. FATH ELAHZADEH, A. MIRSALEHIAN, B. KAZEMI, H. ARSHADI, and B. POURAKBARI, “DETECTION OF CHLAMYDIA TRACHOMATIS AND NEISSERIA GONORRHOAE BY PCR AND MULTIPLEX PCR FROM NON-INVASIVE GENITO-URINARY SPECIMEN OF PATIENTS WITH URETHRITIS,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 62, no. 6, pp. 449–456, 2004, [Online]. Available: https://sid.ir/paper/38812/en

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