ISOLATION OF RHIZOGENIC AGROBACTERIUM TUMEFACIENS STRAIN FROM APPLE TREES

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SALEHI SANAZ; RAZAVI MOHAMMAD; GHASEMI A.A.GH.; REZAEIAN V.; RAHIMIAN H.A.; SHEKARI AZAM

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Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2007 | Volume:43 | Issue:4 (172) Page(s): 159-159

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Title

ISOLATION OF RHIZOGENIC AGROBACTERIUM TUMEFACIENS STRAIN FROM APPLE TREES

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Abstract

Stunting and declining symptoms on 3-5 year old apple trees grafted on Maling rootstocks were associated with uneven growth of root and presence of numerous thin and tender roots, similar to hairy root symptoms, on the main and lateral roots of the trees. The growth of the main root appeared to be arrested. Symptoms were observed on trees grown in two orchards in Semirom in Isfahan province and on apples grafted on Maling-Morton (MM) rootstocks in a nursery in Mashhad, Khorasan province. Segments of roots with hairyroot symptoms were thoroughly washed under tap water, rinsed in sterile distilled water (sDw), and then minced with a sterile razor blade in a few drops of SDW in a Petri plate. The suspension, following a 20-30 min standing at room temperature, was streaked onto plates of 2E and potato dextrose agar + calcium carbonate media and incubated at 25-27°C. White mucoid colonies appearing on both media after 4-5 days of incubation were isolated and used in phenotypic and genotypic characterization and identification. All isolates induced formation of copious amounts of callus on carrot root slices, but none produced any noticeable number of roots on the slices, up to one month post-inoculation. Twenty five of the 60 strains isolated were used as representatives in further characterization tests. The strains were motile, oxidase positive, and catalase positive, could grow on media containing 2% sodium chloride and at 33°C. They formed pellicle on ferric ammonium citrate and used sucrose and citrate as the sole carbon sources for growth. None produced 3-ketolactose nor utilized melezitose or malonate. In polymerase chain reaction (PCR) assays, with ipt and vir D primers, the species-specific fragments of 427 bp and 224 bp long were amplified, respectively, using from DNAs of all strains as templates. Based on the results of phenotypic tests and PCR assays, the strains belong to Agrobacterium tumefaciens (Rhizobium radiobacter). Similar root-inducing strain of A. tumefaciens have also been isolated from cucumber roots in England and named rhizogenic A. tumefaciens .

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APA: Copy

SALEHI, SANAZ, RAZAVI, MOHAMMAD, GHASEMI, A.A.GH., REZAEIAN, V., RAHIMIAN, H.A., & SHEKARI, AZAM. (2007). ISOLATION OF RHIZOGENIC AGROBACTERIUM TUMEFACIENS STRAIN FROM APPLE TREES. IRANIAN JOURNAL OF PLANT PATHOLOGY, 43(4 (172)), 159-159. SID. https://sid.ir/paper/389748/en

Vancouver: Copy

SALEHI SANAZ, RAZAVI MOHAMMAD, GHASEMI A.A.GH., REZAEIAN V., RAHIMIAN H.A., SHEKARI AZAM. ISOLATION OF RHIZOGENIC AGROBACTERIUM TUMEFACIENS STRAIN FROM APPLE TREES. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2007;43(4 (172)):159-159. Available from: https://sid.ir/paper/389748/en

IEEE: Copy

SANAZ SALEHI, MOHAMMAD RAZAVI, A.A.GH. GHASEMI, V. REZAEIAN, H.A. RAHIMIAN, and AZAM SHEKARI, “ISOLATION OF RHIZOGENIC AGROBACTERIUM TUMEFACIENS STRAIN FROM APPLE TREES,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 43, no. 4 (172), pp. 159–159, 2007, [Online]. Available: https://sid.ir/paper/389748/en

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