Investigation of Synergism of Silver Nanoparticle and Erythromycin Inhibition and Detection of Exotoxin-A Gene in Pseudomonas aeruginosa Isolated from Burn Wounds Secretion

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Dehghan Danial; FASIHI RAMANDI MAHDI; TAHERI RAMEZAN ALI

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Journal: Iranian Journal of Medical Microbiology Year:2020 | Volume:14 | Issue:4 Page(s): 379-387

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Title

Investigation of Synergism of Silver Nanoparticle and Erythromycin Inhibition and Detection of Exotoxin-A Gene in Pseudomonas aeruginosa Isolated from Burn Wounds Secretion

Author(s)

Dehghan Danial | FASIHI RAMANDI MAHDI | TAHERI RAMEZAN ALI | Issue Writer Certificate

Keywords

Silver nano particlesQ1

Pseudomonas aeruginosaQ1

ErythromycinQ3

Minimum inhibitory concentration (MIC)Q1

Exotoxin AQ1

Abstract

Background: Pseudomonas aeruginosa infections are resistant to antimicrobial agents and produce toxic virulence factors such as Exotoxin A. Studies have shown that some nanoparticle compounds and antibiotics have a synergistic effect. Therefore, the aim of this study was to investigate the synergistic effect of silver nanoparticles and Erythromycin on antibiotic-resistant P. aeruginosa. Materials & Methods: In this descriptive cross-sectional study, 40 cultured samples of burn wound secretions were taken from Imam Musa Kazem (PBUH) Burns Hospital in Isfahan, Iran. Diagnostic and differential tests were performed. Antibiogram was performed to obtain the bacterial resistance pattern and the Exotoxin A gene was detected by PCR. The bacterial minimum inhibitory concentration (MIC) was then applied to the silver nanoparticles (shape and mean size) and Erythromycin separately and a common mixture of both in 10 dilutions to investigate the synergistic effect. Results & Conclusion: A number of 26 bacteria were strains of P. aeruginosa. Of samples, 25 (96. 15%) had Exotoxin A gene. All samples were sensitive to all Erythromycin concentrations. The mean MIC of nanoparticles against bacteria was reported to be 2 μ g/mL. A solution of 40 μ g/mL Erythromycin and 2 μ g/mL nanoparticles was also considered as MIC solution. Pseudomonas aeruginosa is sensitive to Erythromycin to very low concentrations of silver particles. But no synergistic effect between silver nanoparticles and Erythromycin was reported for this bacterium. Based on PCR results and antibiotic resistance pattern, a significant number of the samples contained the Exotoxin A gene and the use of Erythromycin alone was not appropriate for treatment.

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APA: Copy

Dehghan, Danial, FASIHI RAMANDI, MAHDI, & TAHERI, RAMEZAN ALI. (2020). Investigation of Synergism of Silver Nanoparticle and Erythromycin Inhibition and Detection of Exotoxin-A Gene in Pseudomonas aeruginosa Isolated from Burn Wounds Secretion. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 14(4 ), 379-387. SID. https://sid.ir/paper/391753/en

Vancouver: Copy

Dehghan Danial, FASIHI RAMANDI MAHDI, TAHERI RAMEZAN ALI. Investigation of Synergism of Silver Nanoparticle and Erythromycin Inhibition and Detection of Exotoxin-A Gene in Pseudomonas aeruginosa Isolated from Burn Wounds Secretion. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2020;14(4 ):379-387. Available from: https://sid.ir/paper/391753/en

IEEE: Copy

Danial Dehghan, MAHDI FASIHI RAMANDI, and RAMEZAN ALI TAHERI, “Investigation of Synergism of Silver Nanoparticle and Erythromycin Inhibition and Detection of Exotoxin-A Gene in Pseudomonas aeruginosa Isolated from Burn Wounds Secretion,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 14, no. 4 , pp. 379–387, 2020, [Online]. Available: https://sid.ir/paper/391753/en

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