PREPARATION OF CONJUGATE VACCINE OF HAEMOPHILUS INFLUENZAE TYPE B POLYRIBOSYLRIBITOL PHOSPHATE WITH KEYHOLE LIMPET HEMOCYANIN PROTEIN AND POLY LACTIC-CO-GLYCOLIC ACID NANOPARTICLE AND ITS IMMUNOLOGICAL EVALUATION IN ANIMAL MODEL

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

YAVARI H.; SIADAT S.D.; SHAPOURI R.; SHAFIEE ARDESTANI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2013 | Volume:16 | Issue:2 (71) Page(s): 105-112

Download Full-Text

Persian Verion

View:

2,460

Download:

Cites:

Information Journal Paper

Title

PREPARATION OF CONJUGATE VACCINE OF HAEMOPHILUS INFLUENZAE TYPE B POLYRIBOSYLRIBITOL PHOSPHATE WITH KEYHOLE LIMPET HEMOCYANIN PROTEIN AND POLY LACTIC-CO-GLYCOLIC ACID NANOPARTICLE AND ITS IMMUNOLOGICAL EVALUATION IN ANIMAL MODEL

Author(s)

YAVARI H. | SIADAT S.D. | SHAPOURI R. | SHAFIEE ARDESTANI M. | Issue Writer Certificate

Keywords

CONJUGATE ANTIGENQ2

HAEMOPHILUS INFLUENZAQ2

MENINGITISQ3

VACCINEQ2

Abstract

Background: Today, the use of nano-materials is one of the most common methods of making modern medications; these materials are very useful in increasing the accessibility of drugs to target.The aim of this study is to obtain immunogenic nano-vaccine against MENINGITIS caused by HAEMOPHILUS INFLUENZA.Materials and Methods: In this experimental study, the PRP (polyribosylribitol phosphate) antigen of HAEMOPHILUS INFLUENZA was conjugated to keyhole limpet hemocyanin (KLH), a powerful immunogen molecule, and a nanoparticle with high adsorption called poly lactic co-glycolic acid (PLGA). The two-part and three-part conjugated antigens were injected into male SW1 race mice. The animals were randomly divided into five groups, which received PRP, PRP+KLH, PRP-KLH, PRPKLH- PLGA, and PRP-TT intramuscularly together with complete Freund’s adjuvant, respectively.Twenty eight days after injection, blood samples were obtained and increases in serum antibody titer were determined with ELISA technique. For evaluation of the amount of the produced antigen cell entrance into immune cells, immune cells uptake assay and flow cytometry technique were used.Results: The results showed increases in serum IgG antibody titers of animals immunized with conjugate VACCINEs. The findings also suggest the higher phagocytosis of conjugated triplex-containing nanoparticle by host immune cells.Conclusion: The findings of this study indicate that antigen-containing PLGA has considerably higher absorption and immunogenicity and can be more powerful VACCINEs against Haemophilus MENINGITIS.

Cites

No record.

References

Cite

APA: Copy

YAVARI, H., SIADAT, S.D., SHAPOURI, R., & SHAFIEE ARDESTANI, M.. (2013). PREPARATION OF CONJUGATE VACCINE OF HAEMOPHILUS INFLUENZAE TYPE B POLYRIBOSYLRIBITOL PHOSPHATE WITH KEYHOLE LIMPET HEMOCYANIN PROTEIN AND POLY LACTIC-CO-GLYCOLIC ACID NANOPARTICLE AND ITS IMMUNOLOGICAL EVALUATION IN ANIMAL MODEL. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 16(2 (71)), 105-112. SID. https://sid.ir/paper/392921/en

Vancouver: Copy

YAVARI H., SIADAT S.D., SHAPOURI R., SHAFIEE ARDESTANI M.. PREPARATION OF CONJUGATE VACCINE OF HAEMOPHILUS INFLUENZAE TYPE B POLYRIBOSYLRIBITOL PHOSPHATE WITH KEYHOLE LIMPET HEMOCYANIN PROTEIN AND POLY LACTIC-CO-GLYCOLIC ACID NANOPARTICLE AND ITS IMMUNOLOGICAL EVALUATION IN ANIMAL MODEL. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2013;16(2 (71)):105-112. Available from: https://sid.ir/paper/392921/en

IEEE: Copy

H. YAVARI, S.D. SIADAT, R. SHAPOURI, and M. SHAFIEE ARDESTANI, “PREPARATION OF CONJUGATE VACCINE OF HAEMOPHILUS INFLUENZAE TYPE B POLYRIBOSYLRIBITOL PHOSPHATE WITH KEYHOLE LIMPET HEMOCYANIN PROTEIN AND POLY LACTIC-CO-GLYCOLIC ACID NANOPARTICLE AND ITS IMMUNOLOGICAL EVALUATION IN ANIMAL MODEL,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 16, no. 2 (71), pp. 105–112, 2013, [Online]. Available: https://sid.ir/paper/392921/en

Related Journal Papers