ESTABLISHMENT, CHARACTERIZATION AND DRUG SENSITIVITY OF A NEW EWING’S SARCOMA CELL LINE (SS-ES-1)

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BADINLOU M.; RAJABALIAN SAEID; POURABOULI IRAN; ESKANDARI HOSSEIN; JANGI AGHDAM H.; HORI MITRA

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Journal: Tehran University Medical Journal Year:2008 | Volume:66 | Issue:4 Page(s): 242-250

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Title

ESTABLISHMENT, CHARACTERIZATION AND DRUG SENSITIVITY OF A NEW EWING’S SARCOMA CELL LINE (SS-ES-1)

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Keywords

EWING’S SARCOMAQ3

CELL LINEQ4

CYTOKERATINQ3

NEUROFILAMENTQ3

CD99Q3

P53Q3

Abstract

Background: EWING’S SARCOMA is one of the most malignant tumors in children and young adults. Only a few established CELL LINEs of EWING’S SARCOMA have been reported, which makes it difficult to study the biological features of these tumors. We have recently established a new EWING’S SARCOMA CELL LINE designated SS-ES-1, originating from a thoracic tumor of a 16-year-old female patient. The SS-ES-1 cells have been grown continuously in culture for over 90 passages. In this report, some characteristics of SS-ES-1 cells are presented.Methods: The cells were grown in DMEM medium supplemented with 10% fetal bovine serum (FBS), 100 mg/ml streptomycin and 100 U/ml penicillin in a humidified atmosphere with 7% CO2 at 37 1/4C. The cells were immunocytochemically characterized using a panel of monoclonal and polyclonal antibodies. Furthermore, the chemo-sensitivity of this CELL LINE to some anticancer drugs was assessed using MTT assay and IC50 values were determined. Results: Morphologically, the SS-ES-1 CELL LINE is composed of poorly differentiated small round cells growing in a multilayer pattern. The immunocytochemical staining demonstrates strong reactivity to CD99, CYTOKERATIN, NEUROFILAMENT, P53 and Ki67 proteins, but no reactivity to GFAP. Based on IC50 values, SS-ES-1 cells display considerable sensitivity to vinblastine (2±0.7 pM), followed by vincristine (0.3±0.12 nM), doxorubicin (0.05±0.03 mM), etoposide (0.64±0.28 mM) and cisplatin (0.67±0.45 mM). Conclusions: In conclusion, the SS-ES-1 CELL LINE demonstrates unique cellular properties, which make it a useful model for studying various aspects of the biology of EWING’S SARCOMA.

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APA: Copy

BADINLOU, M., RAJABALIAN, SAEID, POURABOULI, IRAN, ESKANDARI, HOSSEIN, JANGI AGHDAM, H., & HORI, MITRA. (2008). ESTABLISHMENT, CHARACTERIZATION AND DRUG SENSITIVITY OF A NEW EWING’S SARCOMA CELL LINE (SS-ES-1). TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 66(4), 242-250. SID. https://sid.ir/paper/39360/en

Vancouver: Copy

BADINLOU M., RAJABALIAN SAEID, POURABOULI IRAN, ESKANDARI HOSSEIN, JANGI AGHDAM H., HORI MITRA. ESTABLISHMENT, CHARACTERIZATION AND DRUG SENSITIVITY OF A NEW EWING’S SARCOMA CELL LINE (SS-ES-1). TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2008;66(4):242-250. Available from: https://sid.ir/paper/39360/en

IEEE: Copy

M. BADINLOU, SAEID RAJABALIAN, IRAN POURABOULI, HOSSEIN ESKANDARI, H. JANGI AGHDAM, and MITRA HORI, “ESTABLISHMENT, CHARACTERIZATION AND DRUG SENSITIVITY OF A NEW EWING’S SARCOMA CELL LINE (SS-ES-1),” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 66, no. 4, pp. 242–250, 2008, [Online]. Available: https://sid.ir/paper/39360/en

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