PRODUCTION OF RECOMBINANT CAGA PROTEIN OF HELICOBACTER PYLORI

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FARJADI V.; ABTAHI H.; ZOLFAGHARI M.R.; SOUFIAN S.; HASANZADEH L.

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Journal: Journal of Arak University Medical Sciences Year:2013 | Volume:16 | Issue:7 (76) Page(s): 36-44

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Title

PRODUCTION OF RECOMBINANT CAGA PROTEIN OF HELICOBACTER PYLORI

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Keywords

CLONINGQ3

CAGA PROTEINQ2

HELICOBACTER PYLORIQ2

RECOMBINANT PROTEINSQ2

Abstract

Background: HELICOBACTER PYLORI (H. pylori) is a gram negative bacilli that causes the stomach and duodenum diseases in human. An important virulence factor of H. pylori is CagA gene which increases the colonization in stomach epithelial cells and lead to inflammation and peptic ulcers. The aim of the present study was to produce recombinant protein containing highly antigenic region of CagA in E. coli.Materials and Methods: In this experimental study, the antigenic region of CagA gene with 1245 base pair was detected by bioinformatics methods, proliferated by PCR method, digested by BamHI and XhoI restriction enzymes and cloned into pET32a plasmid and was expressed in theE. coli BL21 (DE3) pLysSwith induction by IPTG. The expressed protein was purified with Ni-NTA kit and its antigenicity was studied by western blotting method.Results: Data showed the successful CLONING and expression of the target gene. In western blotting, the produced protein interacted with infected human and mice sera.Conclusion: Results indicated that recombinant CAGA PROTEIN (65 KDa) maintained its antigenicity, so could be used for serological diagnosis of H. pylori diseases and production of vaccine.

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APA: Copy

FARJADI, V., ABTAHI, H., ZOLFAGHARI, M.R., SOUFIAN, S., & HASANZADEH, L.. (2013). PRODUCTION OF RECOMBINANT CAGA PROTEIN OF HELICOBACTER PYLORI. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 16(7 (76)), 36-44. SID. https://sid.ir/paper/393662/en

Vancouver: Copy

FARJADI V., ABTAHI H., ZOLFAGHARI M.R., SOUFIAN S., HASANZADEH L.. PRODUCTION OF RECOMBINANT CAGA PROTEIN OF HELICOBACTER PYLORI. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2013;16(7 (76)):36-44. Available from: https://sid.ir/paper/393662/en

IEEE: Copy

V. FARJADI, H. ABTAHI, M.R. ZOLFAGHARI, S. SOUFIAN, and L. HASANZADEH, “PRODUCTION OF RECOMBINANT CAGA PROTEIN OF HELICOBACTER PYLORI,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 16, no. 7 (76), pp. 36–44, 2013, [Online]. Available: https://sid.ir/paper/393662/en

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