ISOLATION , VEROTOXIN ASSAY AND SEROGROUPING OF ENTEROHEMORRHAGIC E. COLI (EHEC) STRAINS IN ILAM PROVINCE (AUTUMN, 1999)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SADEGHIFARD NOUR KHODA; AZIZI JALILIANE F.; ASLANI M.M.; SHIKHIANE A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2002 | Volume:10 | Issue:1 Page(s): 48-53

Download Full-Text

Persian Verion

View:

745

Download:

Cites:

Information Journal Paper

Title

ISOLATION , VEROTOXIN ASSAY AND SEROGROUPING OF ENTEROHEMORRHAGIC E. COLI (EHEC) STRAINS IN ILAM PROVINCE (AUTUMN, 1999)

Author(s)

SADEGHIFARD NOUR KHODA | AZIZI JALILIANE F. | ASLANI M.M. | SHIKHIANE A. | Issue Writer Certificate

Keywords

ENTEROHEMORRHAGIC E.COLIQ3

VEROTOXINQ4

HEMOLYTIC UREMIC SYNDROMEQ4

HEMORRHAGIC COLITIS.Q3

Abstract

Verotoxin producing E.coli belonging to several different "0" serotype, have been associated closely with HEMOLYTIC UREMIC SYNDROME (HUS) and HEMORRHAGIC COLITIS.(HC). In order to isolate EHEC and carry out VEROTOXIN assays of EHEC strains in Ilam Province 1557 faecal samples were collected- stool samples were cultured for initial isolation of EHEC on sorbitol Macconkey agar medium and biochemical tests were used for confirmation of EHEC. The colony sweepy polymyxin-B extraction method was used for extraction of toxin.In this study, 26 isolates of EHEC were isolated and all of them produced a cytopathic effect on Vero cells. No significant difference was seen between the rate of separated EHEC and diarrhea was observed in rural areas and most of the cases who were EHEC positive were normal and had no diarrhea , but this difference was significant in urban areas. (P=0.001). Neutralization assay revealed that the cytopathic effect on vero cells induced by 18 samples(69.2%) could be neutralized with anti-VT1 serum, and 7 (26.9%) with anti-VT2. The effect of 1 sample (3.9%) could only be neutralized with a mixture of anti- VT1 / VT2 sera. In serogrouping test among the 26 isolates, one isolate(3.9%) showed an agglutination reaction with antiserum 0157:H7. The findings indicate that in Ilam, EHEC isolates are present and VT1 are produced by most of the strains.

Cites

No record.

References

No record.

Cite

APA: Copy

SADEGHIFARD, NOUR KHODA, AZIZI JALILIANE, F., ASLANI, M.M., & SHIKHIANE, A.. (2002). ISOLATION , VEROTOXIN ASSAY AND SEROGROUPING OF ENTEROHEMORRHAGIC E. COLI (EHEC) STRAINS IN ILAM PROVINCE (AUTUMN, 1999). JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 10(1), 48-53. SID. https://sid.ir/paper/395126/en

Vancouver: Copy

SADEGHIFARD NOUR KHODA, AZIZI JALILIANE F., ASLANI M.M., SHIKHIANE A.. ISOLATION , VEROTOXIN ASSAY AND SEROGROUPING OF ENTEROHEMORRHAGIC E. COLI (EHEC) STRAINS IN ILAM PROVINCE (AUTUMN, 1999). JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2002;10(1):48-53. Available from: https://sid.ir/paper/395126/en

IEEE: Copy

NOUR KHODA SADEGHIFARD, F. AZIZI JALILIANE, M.M. ASLANI, and A. SHIKHIANE, “ISOLATION , VEROTOXIN ASSAY AND SEROGROUPING OF ENTEROHEMORRHAGIC E. COLI (EHEC) STRAINS IN ILAM PROVINCE (AUTUMN, 1999),” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 10, no. 1, pp. 48–53, 2002, [Online]. Available: https://sid.ir/paper/395126/en

Related Journal Papers