FREQUENCY OF SHIGA TOXIN-PRODUCING STRAINS OF ESCHERICHIA COLI BY MULTIPLEX PCR IN PATIENTS WITH BLOODY DIARRHEA

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MIRSALEHIAN A.; KAMALKHANI R.; KAZEMI B.; FATH ELAHZADEH B.; ALI GHOLI M.; KHALKHALI M.

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Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2005 | Volume:62 | Issue:12 Page(s): 1008-1015

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Title

FREQUENCY OF SHIGA TOXIN-PRODUCING STRAINS OF ESCHERICHIA COLI BY MULTIPLEX PCR IN PATIENTS WITH BLOODY DIARRHEA

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Keywords

STECQ4

MULTIPLEX PCRQ4

BLOODY DIARRHEAQ4

Abstract

Background: Throughqut the world, acute diarrheal diseases are considered as one of the most common causes of death specially among children. The morbidity and mortality associated with gastrointestinal infection and also hemolytic uremic syndrome (HUS) caused by Shiga Toxin-producing E. coli (STEC) has highlighted the threat this organism pose to public health. Methods and Materials: Between April and December 2003, a total of 150 patients with significant BLOODY DIARRHEA attending four medical centers, were screened. All the stool samples were inoculated into the proper culture media according to standard methods. Strains which could not ferment the Sorbitol, were tested by latex agglutination technique using E. coli O157 monovallent antiserum. After DNA extraction from the isolated strains, the MULTIPLEX PCR reaction for both Stx1 & Stx2 genes performed and consequently amplicons were examined by Agarose gel electrophoresis system.Results: This case study demonstrated that three cases (2%) in whole studied population were positive for the presence of STEC. Further analysis showed that in 47 cases (31.3%) other etiologic agents including Shigella and Salmonella species caused the infection.Conclusion: Although it seems that the incidence and prevalence of gastrointestinal infections caused by STEC are low in Iran, the significant complications and disorders due to STEC infection such as HUS, highlight the importance of using rapid, sensitive and specific molecular and serological diagnostic methods.

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APA: Copy

MIRSALEHIAN, A., KAMALKHANI, R., KAZEMI, B., FATH ELAHZADEH, B., ALI GHOLI, M., & KHALKHALI, M.. (2005). FREQUENCY OF SHIGA TOXIN-PRODUCING STRAINS OF ESCHERICHIA COLI BY MULTIPLEX PCR IN PATIENTS WITH BLOODY DIARRHEA. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 62(12), 1008-1015. SID. https://sid.ir/paper/39538/en

Vancouver: Copy

MIRSALEHIAN A., KAMALKHANI R., KAZEMI B., FATH ELAHZADEH B., ALI GHOLI M., KHALKHALI M.. FREQUENCY OF SHIGA TOXIN-PRODUCING STRAINS OF ESCHERICHIA COLI BY MULTIPLEX PCR IN PATIENTS WITH BLOODY DIARRHEA. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2005;62(12):1008-1015. Available from: https://sid.ir/paper/39538/en

IEEE: Copy

A. MIRSALEHIAN, R. KAMALKHANI, B. KAZEMI, B. FATH ELAHZADEH, M. ALI GHOLI, and M. KHALKHALI, “FREQUENCY OF SHIGA TOXIN-PRODUCING STRAINS OF ESCHERICHIA COLI BY MULTIPLEX PCR IN PATIENTS WITH BLOODY DIARRHEA,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 62, no. 12, pp. 1008–1015, 2005, [Online]. Available: https://sid.ir/paper/39538/en

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