EVALUATION OF MODIFIED IMMUNOTURBIDIMETERY METHOD FOR MICROALBUMINURIA DETECTION IN DIABETIC PATIENTS

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KHAZRAEINIA P.; TAHERI MOHAMMAD; TAIEFEH F.; ATIABI N.; BAHONAR A.

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Title

EVALUATION OF MODIFIED IMMUNOTURBIDIMETERY METHOD FOR MICROALBUMINURIA DETECTION IN DIABETIC PATIENTS

Author(s)

Keywords

DIABETQ4

MICROALBUMINURIAQ4

IMMUNOTURBIDMETEREY NEPHROPATHYQ4

Abstract

Introduction: DIABETic nephropathy is a serious late complication in patient with DIABETes mellitus. Thus determination of MICROALBUMINURIA has become an important test for screening incipient nephropathy in DIABETes and in clinical follow up studies. Material and methods: Immunoturbidimetery is a simple and rapid method, based on antigen (albumin) antibody (anti albumin). Turbidity can be measured by common photometer in laboratories and the method is sensitive enough for clinical purpose. After preparation rabbit anti human albumin, some microalbumin samples were prepared by using sensitive scale.Immunoturbidimetery method was done on these samples and the optical densities (OD) were booked and then typical references curve was drawn. Another 27 microalbumin samples were made by sensitive scale (20-200 µg/ml) and their amounts were determined by using lmmunoturbidimetery method and standard curve.Result: By using this method, sensitivity and specificity of test was %92.59 and %96.29 respectively. Several urine, samples were collected from DIABETic patients. Some samples were negative in protein by using urine strip test but these samples were shown microalbumin by Immunoturbidimetery method.Conclusion: In conclusion this method is rapid, cheap and sensitive enough for clinical purpose.

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APA: Copy

KHAZRAEINIA, P., TAHERI, MOHAMMAD, TAIEFEH, F., ATIABI, N., & BAHONAR, A.. (2005). EVALUATION OF MODIFIED IMMUNOTURBIDIMETERY METHOD FOR MICROALBUMINURIA DETECTION IN DIABETIC PATIENTS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 62(12), 1036-1042. SID. https://sid.ir/paper/39539/en

Vancouver: Copy

KHAZRAEINIA P., TAHERI MOHAMMAD, TAIEFEH F., ATIABI N., BAHONAR A.. EVALUATION OF MODIFIED IMMUNOTURBIDIMETERY METHOD FOR MICROALBUMINURIA DETECTION IN DIABETIC PATIENTS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2005;62(12):1036-1042. Available from: https://sid.ir/paper/39539/en

IEEE: Copy

P. KHAZRAEINIA, MOHAMMAD TAHERI, F. TAIEFEH, N. ATIABI, and A. BAHONAR, “EVALUATION OF MODIFIED IMMUNOTURBIDIMETERY METHOD FOR MICROALBUMINURIA DETECTION IN DIABETIC PATIENTS,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 62, no. 12, pp. 1036–1042, 2005, [Online]. Available: https://sid.ir/paper/39539/en

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