Study of cytotoxicity and cell death of an active compound from prazinoic acids in chronic myeloid leukemia K562 cell line

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Beignezhad Halimeh; MOHAMMADZADEH MEHDI; MAHDAVI MAJID; MOSTAFAVI HOSSEIN

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Journal: MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES Year:2019 | Volume:41 | Issue:2 Page(s): 14-21

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Title

Study of cytotoxicity and cell death of an active compound from prazinoic acids in chronic myeloid leukemia K562 cell line

Author(s)

Beignezhad Halimeh | MOHAMMADZADEH MEHDI | MAHDAVI MAJID | MOSTAFAVI HOSSEIN | Issue Writer Certificate

Keywords

ApoptosisQ1

2-DP.PQ1

CytotoxicityQ2

K562 cellQ1

Abstract

Background: Nowadays, more than 100 types of cancer are known worldwide, which leukemia is one of the most common type. Chronic myeloid leukemia (CML) is one of the most recognized forms of leukemia, accounting for between 15% and 20% of total leukemia. Efforts to find new synthetic compounds are one of the basic strategies for finding new drugs for CML treatment. The purpose of this study was to investigate the effects of growth suppression and induction of Apoptosis (family of pyrazinoic acid) on the cancer cell line of K562 leukemia. Methods: In this study, the effect of a certain concentration of 2-oxo-1, 2-diphenylethyl-pirazine-2-carboxylate (2 DP. P) was evaluated on the growth, cell cycle and induction of Apoptosis of K562 cells using various techniques. MTT test was used to investigate the effects of 2-DP. P on cell viability. For this purpose, 5×104 of K562 cells were cultured at concentrations of 25-100 μ M from the 2-DP. P for 24, 48 and 72 hours intervals. Electrophoresis, fluorescence microscopy and flow cytometry were used for analysis of Apoptosis. Results: The 2-DP. P inhibited dose and time dependently growth in K562 cells. IC50 of the compound was calculated 25 μ M. Also, data from fluorescence microscopy, DNA fragmentation and cell cycle analysis confirmed the reduction of viability and induction of Apoptosis by the compound. Conclusion: due to the effects of growth inhibition and induction of Apoptosis by the 2-DP. P, this compound can be suggested as a new and effective compound for further studies in treating patients with leukemia.

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APA: Copy

Beignezhad, Halimeh, MOHAMMADZADEH, MEHDI, MAHDAVI, MAJID, & MOSTAFAVI, HOSSEIN. (2019). Study of cytotoxicity and cell death of an active compound from prazinoic acids in chronic myeloid leukemia K562 cell line. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, 41(2 ), 14-21. SID. https://sid.ir/paper/396292/en

Vancouver: Copy

Beignezhad Halimeh, MOHAMMADZADEH MEHDI, MAHDAVI MAJID, MOSTAFAVI HOSSEIN. Study of cytotoxicity and cell death of an active compound from prazinoic acids in chronic myeloid leukemia K562 cell line. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;41(2 ):14-21. Available from: https://sid.ir/paper/396292/en

IEEE: Copy

Halimeh Beignezhad, MEHDI MOHAMMADZADEH, MAJID MAHDAVI, and HOSSEIN MOSTAFAVI, “Study of cytotoxicity and cell death of an active compound from prazinoic acids in chronic myeloid leukemia K562 cell line,” MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, vol. 41, no. 2 , pp. 14–21, 2019, [Online]. Available: https://sid.ir/paper/396292/en

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