Isolation and identification of hydrolytic enzymes generated by halophilic bacteria in center of Iran

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Shadi Mohammad; HEYDARI HAMIDREZA; ELYASIFAR BABAK; Dilmaghani Azita

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Journal: MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES Year:2019 | Volume:41 | Issue:5 Page(s): 65-71

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Title

Isolation and identification of hydrolytic enzymes generated by halophilic bacteria in center of Iran

Author(s)

Shadi Mohammad | HEYDARI HAMIDREZA | ELYASIFAR BABAK | Dilmaghani Azita | Issue Writer Certificate

Keywords

PectinaseQ1

AmylaseQ3

LipaseQ2

Halophilic bacteriaQ1

Abstract

Background: Bacteria are one of the important sources of producing enzymes. The aim of this study was to the isolate the Halophilic bacteria producing Amylase, Lipase and Pectinase from saline soil of the center of Iran. Methods: In this study, soil samples were collected from Dagh Biarjmand and Haj Aligholi in Semnan province. At first, isolated Halophilic bacteria were cultured and screened for producing of hydrolytic enzymes including Amylase, Lipase and Pectinase using enzyme-specific media. The enzymatic activities of isolates were recognized by induction of growth inhibition zone and/or precipitation around colonies after addition of related indicators. Afterward, enzyme positive isolates were identifies using the 16S rRNA sequencing. Results: Out of 7 bacterial isolates, 4 bacteria with Amylase activity, 6 bacteria with Lipase activity and 3 bacteria with Pectinase activity were recognized and identified. Results showed that 2 isolates with the ability of producing all three types of enzymes belong to Bacillus genus using molecular identification methods. Furthermore, the Halophilic bacteria producing Amylase and Pectinase were belonging to Bacillus and Halobacillus genera. Besides, bacteria producing Lipase belonged to Bacillus and Virgibacillus genera. Conclusion: The Bacillus bacteria isolated from the soil can produce all three Amylase, Pectinase and Lipase enzymes.

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References

Virgibacillus ainsalahensis sp. nov., a Moderately Halophilic Bacterium Isolated from Sediment of a Saline Lake in South of Algeria

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APA: Copy

Shadi, Mohammad, HEYDARI, HAMIDREZA, ELYASIFAR, BABAK, & Dilmaghani, Azita. (2019). Isolation and identification of hydrolytic enzymes generated by halophilic bacteria in center of Iran. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, 41(5 ), 65-71. SID. https://sid.ir/paper/396320/en

Vancouver: Copy

Shadi Mohammad, HEYDARI HAMIDREZA, ELYASIFAR BABAK, Dilmaghani Azita. Isolation and identification of hydrolytic enzymes generated by halophilic bacteria in center of Iran. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;41(5 ):65-71. Available from: https://sid.ir/paper/396320/en

IEEE: Copy

Mohammad Shadi, HAMIDREZA HEYDARI, BABAK ELYASIFAR, and Azita Dilmaghani, “Isolation and identification of hydrolytic enzymes generated by halophilic bacteria in center of Iran,” MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, vol. 41, no. 5 , pp. 65–71, 2019, [Online]. Available: https://sid.ir/paper/396320/en

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