MIR-520D EXPRESSION ANALYSIS IN BREAST CANCER

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SAVAD SHAHRAM; MEHDIPOR PARVIN; SHIRDAST HODA; NEKOOHESH LADAN; NEKOOHESH LEILI; SHIRKOOHI REZA; NIKOUI VAHID; MIRYOUNESI MOHAMMAD; MODARESI MOHAMMAD HOSSEIN

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2013 | Volume:71 | Issue:2 Page(s): 90-95

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,150

Download:

Cites:

Information Journal Paper

Title

MIR-520D EXPRESSION ANALYSIS IN BREAST CANCER

Author(s)

Keywords

BREAST CANCERQ2

MIR-520DQ2

REAL TIME RT-PCRQ2

SYBR GREEN I DYEQ2

Abstract

Background: BREAST CANCER is the most common cancer in women. Non-coding RNAs especially miRNAs have important regulatory roles in cancer. MiRNAs are 21-24 nucleotides which have different levels of expression between tumors and normal tissues. In this study, we have analyzed expression level of MIR-520D in three different groups of BREAST CANCER.Methods: Fifty nine samples were divided into different groups according to their immunohistochemistry (IHC) classification: estrogen receptor (ER) positive and/or progesterone receptor (PR) positive group (as group I); human epidermal growth factor receptor 2 (HER2) positive group (as group II); and Triple negative group (as group III).After small RNA extraction from tissues, cDNAs were synthesized and Real time RTPCR carried out using DNA binding dye. Expression levels were analyzed by LinRegPCR and REST software.Results: MIR-520D under- expressed in all of three different groups. The expression ratio in groups I, II, and III were 0.193, 0.167, 0.21, respectively, but only the result from group II was significant (P=0.017). According to the different clinicopathological status of BREAST CANCER, MIR-520D underexpressed significantly not only in patients with metastatic lymph node (P=0.019) but also in patients which have cancer at stage III (P=0.036).Conclusion: In this study, we found that MIR-520D possibly acts as a tumor suppressor.It may be useful for diagnosis of tumor from normal tissue. In addition, MIR-520D significantly underexpressed in HER-2 positive group of BREAST CANCERs. Therefore, it may be useful as an additional diagnostic test in this group of breast tumors along with other biomarkers.

Cites

No record.

References

No record.

Cite

APA: Copy

SAVAD, SHAHRAM, MEHDIPOR, PARVIN, SHIRDAST, HODA, NEKOOHESH, LADAN, NEKOOHESH, LEILI, SHIRKOOHI, REZA, NIKOUI, VAHID, MIRYOUNESI, MOHAMMAD, & MODARESI, MOHAMMAD HOSSEIN. (2013). MIR-520D EXPRESSION ANALYSIS IN BREAST CANCER. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 71(2), 90-95. SID. https://sid.ir/paper/39641/en

Vancouver: Copy

SAVAD SHAHRAM, MEHDIPOR PARVIN, SHIRDAST HODA, NEKOOHESH LADAN, NEKOOHESH LEILI, SHIRKOOHI REZA, NIKOUI VAHID, MIRYOUNESI MOHAMMAD, MODARESI MOHAMMAD HOSSEIN. MIR-520D EXPRESSION ANALYSIS IN BREAST CANCER. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2013;71(2):90-95. Available from: https://sid.ir/paper/39641/en

IEEE: Copy

SHAHRAM SAVAD, PARVIN MEHDIPOR, HODA SHIRDAST, LADAN NEKOOHESH, LEILI NEKOOHESH, REZA SHIRKOOHI, VAHID NIKOUI, MOHAMMAD MIRYOUNESI, and MOHAMMAD HOSSEIN MODARESI, “MIR-520D EXPRESSION ANALYSIS IN BREAST CANCER,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 71, no. 2, pp. 90–95, 2013, [Online]. Available: https://sid.ir/paper/39641/en

Related Journal Papers