Investigating the Possibility of the Listeria Monocytogenes Entering Into a Viable But Non-Culturable (VBNC) Form and Expression of the Pathogenic Genes During the Frozen Storage of (-18º C) Rainbow Trout Fish Nugget

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Kalteh Sona; Ojagh Seyyed Mahdi; Tabarrayi Alijan; ZOLFAGHARI MAHDI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2019 | Volume:13 | Issue:1 Page(s): 69-79

Download Full-Text

Persian Verion

View:

399

Download:

Cites:

Information Journal Paper

Title

Investigating the Possibility of the Listeria Monocytogenes Entering Into a Viable But Non-Culturable (VBNC) Form and Expression of the Pathogenic Genes During the Frozen Storage of (-18º C) Rainbow Trout Fish Nugget

Author(s)

Kalteh Sona | Ojagh Seyyed Mahdi | Tabarrayi Alijan | ZOLFAGHARI MAHDI | Issue Writer Certificate

Keywords

Listeria monocytogenesQ1

VBNCQ1

hlyQ1

inlAQ1

FreezingQ1

Abstract

Background and Aims: The purpose of this study was to investigate the possibility of Listeria monocytogenes entering the VBNC state during the frozen storage and the expression of its pathogenic genes Materials and Methods: Bacteria in 106 Colony counts of in mid log phase were inoculated into three Culture medium including Normal Saline (NS), BHI Broth and Fish Broth (FB) and kept at-18º Cfor 2 months and examined. Then, bacteria were evaluated on enriched medium BHI agar using culture methods (colony count) over times 4 and 8 hours, 2, 4, 8, 20, 30 and 60 days after the Freezing shock using the method of RT-PCR for investigating the expression of 16S rRNA, hly and inlA genes; they were evaluated before and after the Freezing shock. Results: This bacterium retained its ability to cultivate until the end of the shock, but reduced its number. Freezing stopped the expression of genes of hly and inlA, as these genes were not expressed in a rich culture medium either. By adding blood to the rich culture medium of this bacterium, only the hemolysin O pathogen gene was expressed. Conclusion: Although Freezing does not lead to the introduction of this bacterium into the VBNC state, it is effective as an adverse environmental factor for the bacteria in the expression of its pathogenic genes. Blood and its agents can act as an agent for the induction and clarification of the hly gene, and the expression of pathogenic bacterial genes are independent of each other.

Cites

No record.

References

The Effect of Stressful Conditions on Culturability of Listeria monocytogenes in Food Matrix

Cite

APA: Copy

Kalteh, Sona, Ojagh, Seyyed Mahdi, Tabarrayi, Alijan, & ZOLFAGHARI, MAHDI. (2019). Investigating the Possibility of the Listeria Monocytogenes Entering Into a Viable But Non-Culturable (VBNC) Form and Expression of the Pathogenic Genes During the Frozen Storage of (-18º C) Rainbow Trout Fish Nugget. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 13(1 ), 69-79. SID. https://sid.ir/paper/396437/en

Vancouver: Copy

Kalteh Sona, Ojagh Seyyed Mahdi, Tabarrayi Alijan, ZOLFAGHARI MAHDI. Investigating the Possibility of the Listeria Monocytogenes Entering Into a Viable But Non-Culturable (VBNC) Form and Expression of the Pathogenic Genes During the Frozen Storage of (-18º C) Rainbow Trout Fish Nugget. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2019;13(1 ):69-79. Available from: https://sid.ir/paper/396437/en

IEEE: Copy

Sona Kalteh, Seyyed Mahdi Ojagh, Alijan Tabarrayi, and MAHDI ZOLFAGHARI, “Investigating the Possibility of the Listeria Monocytogenes Entering Into a Viable But Non-Culturable (VBNC) Form and Expression of the Pathogenic Genes During the Frozen Storage of (-18º C) Rainbow Trout Fish Nugget,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 13, no. 1 , pp. 69–79, 2019, [Online]. Available: https://sid.ir/paper/396437/en

Related Journal Papers