OPTIMIZING ACRIDINE ORANGE STAINING FOR ASSESSMENT OF PROTECTIVE EFFECTS OF SAFFRON AND VITAMIN E ON RAT SPERM DNA STRUCTURE

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RAZAVI SHAHNAZ; VAEZ SAYYED AHMAD; MARDANI MOHAMMAD

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Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2014 | Volume:32 | Issue:293 Page(s): 1-12

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Title

OPTIMIZING ACRIDINE ORANGE STAINING FOR ASSESSMENT OF PROTECTIVE EFFECTS OF SAFFRON AND VITAMIN E ON RAT SPERM DNA STRUCTURE

Author(s)

RAZAVI SHAHNAZ | VAEZ SAYYED AHMAD | MARDANI MOHAMMAD | Issue Writer Certificate

Keywords

ANTIOXIDANTSQ2

SAFFRONQ2

VITAMIN EQ2

DNA DAMAGEQ1

Abstract

Background: In recent decades relation between ROS concentration and semen quality was noted. SAFFRON has traditionally been not only considered as a food additive but also as a medicinal herb, which has a good antioxidant properties. VITAMIN E is considered as a endogenous and supplementary antioxidant which can protects the cell. The aim of this study was to evaluate the protection influence of SAFFRON and VITAMIN E on sperm DNA against acid.Methods: Thirty adult male Wistar rats divided into SAFFRON (n=10), VITAMIN E (n=10) and control (n=10) groups randomly. Rats received SAFFRON (100 mg/kg/day), VITAMIN E (100 mg/kg/day) and distilled water (0.5 cc/day) in SAFFRON, VITAMIN E and control groups respectively. After 60 days, cauda epididymis dissected and sperm were used for analysis of sperm chromatin susceptibility to acid denaturation by acridine orange (AO) staining. AO staining was carried out with or without acid detergent incubation.Findings: Our results purposed that SAFFRON and VITAMIN E can decrease sperm DNA DAMAGE against acid.Conclusion: SAFFRON and VITAMIN E protect DNA against denaturation probably because of their powerful antioxidant properties.

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APA: Copy

RAZAVI, SHAHNAZ, VAEZ, SAYYED AHMAD, & MARDANI, MOHAMMAD. (2014). OPTIMIZING ACRIDINE ORANGE STAINING FOR ASSESSMENT OF PROTECTIVE EFFECTS OF SAFFRON AND VITAMIN E ON RAT SPERM DNA STRUCTURE. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 32(293), 1-12. SID. https://sid.ir/paper/396855/en

Vancouver: Copy

RAZAVI SHAHNAZ, VAEZ SAYYED AHMAD, MARDANI MOHAMMAD. OPTIMIZING ACRIDINE ORANGE STAINING FOR ASSESSMENT OF PROTECTIVE EFFECTS OF SAFFRON AND VITAMIN E ON RAT SPERM DNA STRUCTURE. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2014;32(293):1-12. Available from: https://sid.ir/paper/396855/en

IEEE: Copy

SHAHNAZ RAZAVI, SAYYED AHMAD VAEZ, and MOHAMMAD MARDANI, “OPTIMIZING ACRIDINE ORANGE STAINING FOR ASSESSMENT OF PROTECTIVE EFFECTS OF SAFFRON AND VITAMIN E ON RAT SPERM DNA STRUCTURE,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 32, no. 293, pp. 1–12, 2014, [Online]. Available: https://sid.ir/paper/396855/en

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