Effect of supplementation of lecithin nanoparticles-based extender with reduced glutathione on freezability of bull sperm

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Nadri Touba; ZEINOALDINI SAEED; TOWHIDI ARMIN; RIAZI GHOLAM HOSSEIN; ZHANDI MEHDI; SHARAFI MOHSEN

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Journal: Journal of Animal Production Year:2020 | Volume:22 | Issue:3 Page(s): 471-477

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Title

Effect of supplementation of lecithin nanoparticles-based extender with reduced glutathione on freezability of bull sperm

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Keywords

AntioxidantsQ1

BullQ1

Freeze-thawQ2

SemenQ1

NanomicelleQ1

Abstract

This study was designed to add the reduced glutathione to a lecithin nanoparticle-based extender and evaluate the quality of Bull sperm after freezing and thawing. In the present study, the effect of four different levels of glutathione (0, 1, 2. 5 and 5 mM) in extender-based on lecithin nanoparticles was evaluated. The lecithin nanoparticles were prepared and the particle size was reduced by using a sonicator device. During three weeks, 48 ejaculates from six Holstein Bulls were collected and frozen. Properties evaluated after freezing and thawing were kinetic parameters (CASA), membrane activity (HOST), membrane integrity (eosin-nigrosine) and morphology (Hancock solution). The results showed that using 2. 5 mM glutathione in the extender significantly increased total and progressive motility (63. 38± 1. 5 and 43. 1± 1. 1 respectively, P <0. 05). The results of eosin-nigrosine staining and Host test showed that the highest viability and cell membrane functionality were related to 2. 5 mM treatment (64. 8± 1. 5 and 58. 1± 1. 1 respectively) (P<0. 05). In general, 2. 5 mM glutathione could improve the quality of Bull sperm compared with other concentrations after freezing and thawing process. It seems that the 2. 5 mM glutathione is optimum concentration for Bull extender based on the lecithin nanoparticles.

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APA: Copy

Nadri, Touba, ZEINOALDINI, SAEED, TOWHIDI, ARMIN, RIAZI, GHOLAM HOSSEIN, ZHANDI, MEHDI, & SHARAFI, MOHSEN. (2020). Effect of supplementation of lecithin nanoparticles-based extender with reduced glutathione on freezability of bull sperm. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), 22(3 ), 471-477. SID. https://sid.ir/paper/396939/en

Vancouver: Copy

Nadri Touba, ZEINOALDINI SAEED, TOWHIDI ARMIN, RIAZI GHOLAM HOSSEIN, ZHANDI MEHDI, SHARAFI MOHSEN. Effect of supplementation of lecithin nanoparticles-based extender with reduced glutathione on freezability of bull sperm. JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE)[Internet]. 2020;22(3 ):471-477. Available from: https://sid.ir/paper/396939/en

IEEE: Copy

Touba Nadri, SAEED ZEINOALDINI, ARMIN TOWHIDI, GHOLAM HOSSEIN RIAZI, MEHDI ZHANDI, and MOHSEN SHARAFI, “Effect of supplementation of lecithin nanoparticles-based extender with reduced glutathione on freezability of bull sperm,” JOURNAL OF ANIMAL PRODUCTION (JOURNAL OF AGRICULTURE), vol. 22, no. 3 , pp. 471–477, 2020, [Online]. Available: https://sid.ir/paper/396939/en

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