Isolation, cloning and bioinformatic analysis of cytochrom oxidase C subunit I in Citrus

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ABASI A.; Ghayeb Zamharir M.; TOHIDFAR M.; HASANZADEH N.

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Journal: Journal of Molecular and Cellular Researches Year:2019 | Volume:32 | Issue:4 Page(s): 539-552

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Title

Isolation, cloning and bioinformatic analysis of cytochrom oxidase C subunit I in Citrus

Author(s)

ABASI A. | Ghayeb Zamharir M. | TOHIDFAR M. | HASANZADEH N. | Issue Writer Certificate

Keywords

CloningQ1

cytochrome oxidase CQ1

Mitochondria genome and orangeQ1

Abstract

Cytochrome oxidase is one of a superfamily of proteins which act as the terminal enzymes of respiratory chains. The two main classes are cytochrome c oxidases, and quinol oxidases. There are two catalytic subunits, I and II. Subunit I contains two heme centers. In this study we have isolated the cytochrom oxidase (COX) gene from orange by PCR and clone it by ligation of this product to pGEM-T vector transformed E. coli bacteria. Sequencing analysis shows that open reading frame for this gene that encode a protein with 217 amino acids. Results of phylogenetic analysis showed that this gene in orange has related to Chondrostoma cytochrom oxidase gene (COX). COX protein secrete in membrane and it's extracellular secretion is little. The aim of this study was isolation and Cloning of COX genes from citrus genome and determining of it's characterizations to practical use in the plants.

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APA: Copy

ABASI, A., Ghayeb Zamharir, M., TOHIDFAR, M., & HASANZADEH, N.. (2019). Isolation, cloning and bioinformatic analysis of cytochrom oxidase C subunit I in Citrus. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 32(4 ), 539-552. SID. https://sid.ir/paper/398180/en

Vancouver: Copy

ABASI A., Ghayeb Zamharir M., TOHIDFAR M., HASANZADEH N.. Isolation, cloning and bioinformatic analysis of cytochrom oxidase C subunit I in Citrus. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2019;32(4 ):539-552. Available from: https://sid.ir/paper/398180/en

IEEE: Copy

A. ABASI, M. Ghayeb Zamharir, M. TOHIDFAR, and N. HASANZADEH, “Isolation, cloning and bioinformatic analysis of cytochrom oxidase C subunit I in Citrus,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 32, no. 4 , pp. 539–552, 2019, [Online]. Available: https://sid.ir/paper/398180/en

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