Isolation and molecular identification of isolates of plant growth promoting Pseudomonas and Bacillus

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ZARE ROGHAYEH; dezfulian Mehrooz; AMINI ZAHRA; KARIMI EBRAHIM; SADEGHI AKRAM; Rahmati Razieh

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Journal: Crop Biotechnology Year:2019 | Volume:8 | Issue:24 Page(s): 95-109

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Title

Isolation and molecular identification of isolates of plant growth promoting Pseudomonas and Bacillus

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Bell pepperQ1

cucumberQ3

phytopathogenQ1

rhizobacteriaQ1

tomatoQ3

Abstract

The use of the synergistic effect of nitrogen-fixing bacteria and phosphate solubilizing bacteria is one of the newest biofertilizer production strategies. Rhizosphere soil samples were collected from tomato, cucumber and Bell pepper commercial greenhouses and screened for Pseudomonas and Bacillus using selective media. One hundred and eight Pseudomonas and 92 Bacillus isolates with PGP activities were isolated and characterized. Fifty-three percent of Pseudomonas isolates and 25% of Bacillus were able to grow on N free medium. Ninety-six percent of Pseudomonas isolates were able to solubilize organic phosphate and 70% of them produce IAA, while none of the Bacillus had these two abilities. About 30% of Pseudomonas and Bacillus isolates produces siderophore, although the ratio of orange halo diameter/colony diameter was greater for Pseudomonas. Only five Bacillus and 31 Pseudomonas isolates inhibited the growth of Phytophthora melonis. Based on these results, although PGP isolates of both genera were found in the rhizosphere soil of commercial greenhouses, these bacteria differed in PGP traits. A Pseudomonas isolate, P3-57 and a Bacillus isolate, C1BY-1 with 50% antagonistic activity against P. melonis which differed in PGP traits selected for future study in greenhouse conditions. Phylogenetic analyses of 16S rRNA gene sequence revealed that strains P3-57 and C1BY-1 are closely related to P. putida and B. subtilis respectively.

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APA: Copy

ZARE, ROGHAYEH, dezfulian, Mehrooz, AMINI, ZAHRA, KARIMI, EBRAHIM, SADEGHI, AKRAM, & Rahmati, Razieh. (2019). Isolation and molecular identification of isolates of plant growth promoting Pseudomonas and Bacillus. JOURNAL OF CROP BIOTECHNOLOGY, 8(24 ), 95-109. SID. https://sid.ir/paper/404350/en

Vancouver: Copy

ZARE ROGHAYEH, dezfulian Mehrooz, AMINI ZAHRA, KARIMI EBRAHIM, SADEGHI AKRAM, Rahmati Razieh. Isolation and molecular identification of isolates of plant growth promoting Pseudomonas and Bacillus. JOURNAL OF CROP BIOTECHNOLOGY[Internet]. 2019;8(24 ):95-109. Available from: https://sid.ir/paper/404350/en

IEEE: Copy

ROGHAYEH ZARE, Mehrooz dezfulian, ZAHRA AMINI, EBRAHIM KARIMI, AKRAM SADEGHI, and Razieh Rahmati, “Isolation and molecular identification of isolates of plant growth promoting Pseudomonas and Bacillus,” JOURNAL OF CROP BIOTECHNOLOGY, vol. 8, no. 24 , pp. 95–109, 2019, [Online]. Available: https://sid.ir/paper/404350/en

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