COMPARISON OF ELISA, INDIRECT IMMUNOFLUORESCENCE AND MYCOLOGICAL METHOD FOR DETESTION OF STRONGYLOIDES STERCORALIS INFECTION AMONG HEMODIALYSIS AND RENAL TRANSPLANTATION PATIENTS

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NOUBAKHT HAGHIGHI A.; SADR-NOORI A.

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Journal: Feyz Medical Sciences Journal Year:2000 | Volume:4 | Issue:15 Page(s): 23-31

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Title

COMPARISON OF ELISA, INDIRECT IMMUNOFLUORESCENCE AND MYCOLOGICAL METHOD FOR DETESTION OF STRONGYLOIDES STERCORALIS INFECTION AMONG HEMODIALYSIS AND RENAL TRANSPLANTATION PATIENTS

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NOUBAKHT HAGHIGHI A. | SADR-NOORI A. | Issue Writer Certificate

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Abstract

History and Objectives: Strongyloides stercoralis infection has adverse effect on hemodialysis and renal transplanted patients. Since strongyloides stercoralis infection does not have apparent clinical signs, the present study was carned out to address the diagnostic power of ELISA and indirect immunofluoresence on pateints referred to the hospitals for renal transplantation and hemodialysis in 1996.Materials and Methods: A diagnostic clinical trial was performed on 110 renal transplant and hemodialysis patients. ELISA, indirect immunofluoresence, direct microscopic fecal examination and formalin-ether examination of fecal samples were compared. Sensitivity, specificity and predictive value of each method were determined.Results: From 10 samples, 14 showed infection whereas 96 samples did not show any infection. Diagnostic value was 100% for ELISA and indirect immunofluoresent methods. There were close coorelation between the two mwthods. Both method of direct fecal examination formalin ether method were unable to detect all the positive samples and all negative samples were reported as such by two fecal examination methods. There were no significant difference on the detection of positive samples of hemodialysis patients of Gilan and Tehran province and between emodialysis and renal transplant patients of Gilan province. No significant difference between postive sample and sex was recorded.Conclusions: Immunosuppressant chemotherapy of rena l transplant patients causes reduction of inmmune response and low antibody titer and poor diognostic value of immunologic tests. ELISA and indirect immunofluoresent detection of strongyloides stercoralis infection is very simple and rapid test to perform and has an high degree of sensitivity and specificity.

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APA: Copy

NOUBAKHT HAGHIGHI, A., & SADR-NOORI, A.. (2000). COMPARISON OF ELISA, INDIRECT IMMUNOFLUORESCENCE AND MYCOLOGICAL METHOD FOR DETESTION OF STRONGYLOIDES STERCORALIS INFECTION AMONG HEMODIALYSIS AND RENAL TRANSPLANTATION PATIENTS. FEYZ, 4(15), 23-31. SID. https://sid.ir/paper/40889/en

Vancouver: Copy

NOUBAKHT HAGHIGHI A., SADR-NOORI A.. COMPARISON OF ELISA, INDIRECT IMMUNOFLUORESCENCE AND MYCOLOGICAL METHOD FOR DETESTION OF STRONGYLOIDES STERCORALIS INFECTION AMONG HEMODIALYSIS AND RENAL TRANSPLANTATION PATIENTS. FEYZ[Internet]. 2000;4(15):23-31. Available from: https://sid.ir/paper/40889/en

IEEE: Copy

A. NOUBAKHT HAGHIGHI, and A. SADR-NOORI, “COMPARISON OF ELISA, INDIRECT IMMUNOFLUORESCENCE AND MYCOLOGICAL METHOD FOR DETESTION OF STRONGYLOIDES STERCORALIS INFECTION AMONG HEMODIALYSIS AND RENAL TRANSPLANTATION PATIENTS,” FEYZ, vol. 4, no. 15, pp. 23–31, 2000, [Online]. Available: https://sid.ir/paper/40889/en

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