Investigation of Genetic Structure of Sanjabi Sheep Using Microsatellite Markers

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SEYED SHARIFI R.; Badbarin S.; HEDAYAT EVRIGH N.; Seyed Davati J.; Saver Sofla S.

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Journal: JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES) Year:2020 | Volume:13 | Issue:4 (51) Page(s): 61-70

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Title

Investigation of Genetic Structure of Sanjabi Sheep Using Microsatellite Markers

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Keywords

Genetic structureQ1

Sanjabi sheepQ2

Microsatellite markersQ1

Abstract

Inroduction & Objective: Sanjabi sheep is one of the valuable breeds of sheep in the west of the country, which is very important in terms of meat and wool production. Considering the importance of native sheep breeds and their breeding, the present study was performed in order to achieve more production (quantity and quality), identification of Genetic structure and estimation of relevant parameters (number of observed and effective alleles, heterozygosity, Shannon index, etc. ). Material and Method: The study population consisted of 100 Sanjabi rams and ewes located in Mehregan station of Kermanshah province that were randomly selected. DNA extraction was performed by salt method. PCR reaction was performed using 10 Microsatellite markers. Amplified DNA fragments were stained on acrylamide gel and detected by silver nitrate method. Alleles were scored according to their size and compared to the standard index PUC8 of Fermentase Company. Results: The results of this study showed that all markers were polymorphic. The mean number of observed and effective alleles for all markers was calculated to be 4. 5 and 2. 9012, respectively. The highest and lowest expected heterozygosity were obtained in OarFCB11 and BMS2721 markers equal to 0. 7548 and 0. 5619, respectively. The expected heterozygosity for all markers (genetic diversity) was 0. 6487. Conclusion: According to the obtained results, it can be said that the studied sheep have a desirable level of diversity and can be achieved by high breeding by breeding and selection of superior livestock. The markers used also have a high ability to study the Genetic structure of Sanjabi sheep and their use is recommended in future studies.

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APA: Copy

SEYED SHARIFI, R., Badbarin, S., HEDAYAT EVRIGH, N., Seyed Davati, J., & Saver Sofla, S.. (2020). Investigation of Genetic Structure of Sanjabi Sheep Using Microsatellite Markers. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), 13(4 (51) ), 61-70. SID. https://sid.ir/paper/410979/en

Vancouver: Copy

SEYED SHARIFI R., Badbarin S., HEDAYAT EVRIGH N., Seyed Davati J., Saver Sofla S.. Investigation of Genetic Structure of Sanjabi Sheep Using Microsatellite Markers. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES)[Internet]. 2020;13(4 (51) ):61-70. Available from: https://sid.ir/paper/410979/en

IEEE: Copy

R. SEYED SHARIFI, S. Badbarin, N. HEDAYAT EVRIGH, J. Seyed Davati, and S. Saver Sofla, “Investigation of Genetic Structure of Sanjabi Sheep Using Microsatellite Markers,” JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), vol. 13, no. 4 (51) , pp. 61–70, 2020, [Online]. Available: https://sid.ir/paper/410979/en

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