EVALUATION OF PRESENCE OF THE BLA-SHV AND BLA-AMPC (CITM, FOX) β-LACTAMASE GENES IN CLINICAL ISOLATES OF ESCHERICHIA COLI

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SOLTAN DALLAL MOHAMMAD MAHDI; SABBAGHI AYLAR; FALLAH JALIL; AGHAMIRZAEI HEDROSHA MOLLA; RASTEGAR LARI ABDOLAZIZ; ESHRAGHIAN MOHAMMAD R.; FARD SANEI ATEFEH

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Journal: Journal of Medical Council of Iran Year:2010 | Volume:28 | Issue:3 Page(s): 269-276

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Title

EVALUATION OF PRESENCE OF THE BLA-SHV AND BLA-AMPC (CITM, FOX) β-LACTAMASE GENES IN CLINICAL ISOLATES OF ESCHERICHIA COLI

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Keywords

ESCHERICHIA COLIQ2

EXTENDED SPECTRUM β-LACTAMASES (-TYPE SHV)Q3

AMPC β-LACTAMASEQ3

Abstract

Background: Resistance to beta-lactam antibiotics along with clinical isolates is frequently resulting production of b-lactamase enzymes. In recent years, the production of extended spectrum b-lactamases (ESBLs) and AmpC b-lactamase among clinical isolate especially ESCHERICHIA COLI is greatly increased. On the other hand, beta lactamase genes have several subfamilies, and designing universal primers could be valuable to detect all of them. Therefore, the aim of this study was to survey prevalence of phenotypic ESBLs and detection of SHV and AmpC (CITM, FOX) –type b-lactamase genes by using universal primers through PCR.Methods: A total of 500 clinical samples were collected from hospitals of Tehran and 200 E.coli isolates were detected by standard biochemical tests. Subsequently, these isolates were screened for b-lactamase production by Disk diffusion method and combined disk. Resistant isolates were evaluated for molecular assessing of SHV, CITM and FOX genes by using PCR.Results: Among entire of 200 E.coli, 128 (64%) isolates were selected via phenotypic tests for detection of bla-SHV and bla-AmpC (CITM, FOX) genes via PCR. With 95% confidence, 7 (5.5%) and 13 (10.2%) E.coli harbor bla-SHV and bla-CITM, respectively. Fox gene was not detected in any samples.Conclusion: Results were showed that complete detection of b-lactamase enzymes is essential for resistant control and the appropriate prescription of b-lactam drugs. So using molecular assay with phenotypic test is important.

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APA: Copy

SOLTAN DALLAL, MOHAMMAD MAHDI, SABBAGHI, AYLAR, FALLAH, JALIL, AGHAMIRZAEI, HEDROSHA MOLLA, RASTEGAR LARI, ABDOLAZIZ, ESHRAGHIAN, MOHAMMAD R., & FARD SANEI, ATEFEH. (2010). EVALUATION OF PRESENCE OF THE BLA-SHV AND BLA-AMPC (CITM, FOX) β-LACTAMASE GENES IN CLINICAL ISOLATES OF ESCHERICHIA COLI. JOURNAL OF MEDICAL COUNCIL OF I.R.I., 28(3), 269-276. SID. https://sid.ir/paper/41311/en

Vancouver: Copy

SOLTAN DALLAL MOHAMMAD MAHDI, SABBAGHI AYLAR, FALLAH JALIL, AGHAMIRZAEI HEDROSHA MOLLA, RASTEGAR LARI ABDOLAZIZ, ESHRAGHIAN MOHAMMAD R., FARD SANEI ATEFEH. EVALUATION OF PRESENCE OF THE BLA-SHV AND BLA-AMPC (CITM, FOX) β-LACTAMASE GENES IN CLINICAL ISOLATES OF ESCHERICHIA COLI. JOURNAL OF MEDICAL COUNCIL OF I.R.I.[Internet]. 2010;28(3):269-276. Available from: https://sid.ir/paper/41311/en

IEEE: Copy

MOHAMMAD MAHDI SOLTAN DALLAL, AYLAR SABBAGHI, JALIL FALLAH, HEDROSHA MOLLA AGHAMIRZAEI, ABDOLAZIZ RASTEGAR LARI, MOHAMMAD R. ESHRAGHIAN, and ATEFEH FARD SANEI, “EVALUATION OF PRESENCE OF THE BLA-SHV AND BLA-AMPC (CITM, FOX) β-LACTAMASE GENES IN CLINICAL ISOLATES OF ESCHERICHIA COLI,” JOURNAL OF MEDICAL COUNCIL OF I.R.I., vol. 28, no. 3, pp. 269–276, 2010, [Online]. Available: https://sid.ir/paper/41311/en

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