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Information Journal Paper

Title

Comparison of MTT and Trypan Blue methods in determining the survival of Vero cell line in HSV-1 infection

Pages

  18-26

Keywords

Abstract

 Background and Objectives: Evaluation of cell Survival is important in cellular studies and the result is decisive for the application of the test substance. Different methods are used to evaluate cell Survival and the correlation between methods is important. Here, Vero cell line was used to evaluate the effects of HSV-1 virus on cell Survival. Cell Survival after HSV-1 infection using sensitive colorimetric methods of 3-, 4-, 5-dimethylthiazole-2-yl,-2, 5-diphenyltetrazolium bromide (MTT), and Trypan Blue on Vero cell line was examined. Materials and Methods: A qualitative study was performed on Vero cell line infected with HSV-1 virus with logarithmic dilutions10-1-5 to 10 dilutions and time intervals of 0, 4, 8, 18 and 24 hours and the s in DMEM containing 2% Fetal bovine serum (FBS). Then cell viability by MTT and Trypan tests was examined. The Survival percentage was used to plot the Survival curve of the assays. Results: Different viral dilutions were used to determine the infectious dose of 50% (TCID50) of HSV1virus with invert microscope. Acyclovir at a concentration of 50 mg/mL was compared as drug control for MTT and Trypan Blue. The results of regression analysis showed cell Survival. Linear, positive and significant correlation was observed between the methods that showed a correlation between MTT and Trypan Blue methods (p< 0. 001, r = 0. 9( Conclusions: The results showed that both methods can be used to determine cell Survival and the results of MTT can be confirmed by Trypan Blue method.

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    Cite

    APA: Copy

    ZAMANIAN, M., NOORMOHAMMADI, Z., AKBARZADEH, T., BINESHIAN, F., & SHARIFI, Z.. (2021). Comparison of MTT and Trypan Blue methods in determining the survival of Vero cell line in HSV-1 infection. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 18(1 ), 18-26. SID. https://sid.ir/paper/413461/en

    Vancouver: Copy

    ZAMANIAN M., NOORMOHAMMADI Z., AKBARZADEH T., BINESHIAN F., SHARIFI Z.. Comparison of MTT and Trypan Blue methods in determining the survival of Vero cell line in HSV-1 infection. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2021;18(1 ):18-26. Available from: https://sid.ir/paper/413461/en

    IEEE: Copy

    M. ZAMANIAN, Z. NOORMOHAMMADI, T. AKBARZADEH, F. BINESHIAN, and Z. SHARIFI, “Comparison of MTT and Trypan Blue methods in determining the survival of Vero cell line in HSV-1 infection,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 18, no. 1 , pp. 18–26, 2021, [Online]. Available: https://sid.ir/paper/413461/en

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