CORRELATION STUDY OF PHOSPHOLIPASE C GENES AND PATHOGENICITY OF MYCOBACTERIUM TUBERCULOSIS STRAINS OF THE BEIJING FAMILY AND NON- BEIJING FAMILY

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GOUDARZI HOSSEIN; MIRSAMADI E.A.S.; FARNIA P.; JAHANI SHERAFAT S.

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Journal: Research in Medicine Year:2010 | Volume:34 | Issue:2 Page(s): 128-131

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Title

CORRELATION STUDY OF PHOSPHOLIPASE C GENES AND PATHOGENICITY OF MYCOBACTERIUM TUBERCULOSIS STRAINS OF THE BEIJING FAMILY AND NON- BEIJING FAMILY

Author(s)

GOUDARZI HOSSEIN | MIRSAMADI E.A.S. | FARNIA P. | JAHANI SHERAFAT S. | Issue Writer Certificate

Keywords

PHOSPHOLIPASE CQ2

MDRQ3

BEIJINGQ3

MYCOBACTERIUM TUBERCULOSISQ3

Abstract

Background: The BEIJING strain of MYCOBACTERIUM TUBERCULOSIS is responsible for more than 25% of cases of Tuberculosis in the world, it has a high transmission potential and there is a significant correlation between BEIJING strain and multidrug resistance. In this study we compared the presence of PHOSPHOLIPASE C genes in BEIJING and Nonbeijing strains of MYCOBACTERIUM TUBERCULOSIS.Methods: CTAB method was used to extract DNA from positive culture specimens from patients with pulmonary tuberculosis. Spoligotyping was used to identify BEIJING from non-Beijing strains. Presence of PHOSPHOLIPASE C genes was ascertained by using PCR. Result: Of 200 specimens, 19 were BEIJING strain, (9.5%) and 181 non BEIJING strains, (90.5%). In BEIJING strains, 16 specimens, (84.2%) were positive for plcA, 17 (89.5%) were positive for plcB and 17 (89.5%) were positive for plcC genes and in non BEIJING strains 17 specimens, (9.4%), were positive for plcA, 18 (9.9%), for plcB and 18 (9.9%) for plcC.Conclusion: Majority of BEIJING strains have PHOSPHOLIPASE C genes that may be responsible for the virulence of this strain.

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APA: Copy

GOUDARZI, HOSSEIN, MIRSAMADI, E.A.S., FARNIA, P., & JAHANI SHERAFAT, S.. (2010). CORRELATION STUDY OF PHOSPHOLIPASE C GENES AND PATHOGENICITY OF MYCOBACTERIUM TUBERCULOSIS STRAINS OF THE BEIJING FAMILY AND NON- BEIJING FAMILY. PAJOUHESH DAR PEZESHKI, 34(2), 128-131. SID. https://sid.ir/paper/41597/en

Vancouver: Copy

GOUDARZI HOSSEIN, MIRSAMADI E.A.S., FARNIA P., JAHANI SHERAFAT S.. CORRELATION STUDY OF PHOSPHOLIPASE C GENES AND PATHOGENICITY OF MYCOBACTERIUM TUBERCULOSIS STRAINS OF THE BEIJING FAMILY AND NON- BEIJING FAMILY. PAJOUHESH DAR PEZESHKI[Internet]. 2010;34(2):128-131. Available from: https://sid.ir/paper/41597/en

IEEE: Copy

HOSSEIN GOUDARZI, E.A.S. MIRSAMADI, P. FARNIA, and S. JAHANI SHERAFAT, “CORRELATION STUDY OF PHOSPHOLIPASE C GENES AND PATHOGENICITY OF MYCOBACTERIUM TUBERCULOSIS STRAINS OF THE BEIJING FAMILY AND NON- BEIJING FAMILY,” PAJOUHESH DAR PEZESHKI, vol. 34, no. 2, pp. 128–131, 2010, [Online]. Available: https://sid.ir/paper/41597/en

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