THE STUDY OF ANTIBIOTIC RESISTANCE AMONG KLEBSIELLA PNEUMONIAE AND EXPRESSION LEVEL OF OQXA AND ACRA GENES BY USING REAL-TIME PCR

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SAADATIAN FARIVAR AREZOO; NOWROOZI JAMILEH; ESLAMI GITA; SABOKBAR AZAR; HASHEMI ALI

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Journal: Research in Medicine Year:2016 | Volume:40 | Issue:1 Page(s): 42-48

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Title

THE STUDY OF ANTIBIOTIC RESISTANCE AMONG KLEBSIELLA PNEUMONIAE AND EXPRESSION LEVEL OF OQXA AND ACRA GENES BY USING REAL-TIME PCR

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Keywords

KLEBSIELLA PNEUMONIAEQ1

EFFLUX PUMPSQ1

ANTIMICROBIAL RESISTANCEQ2

REAL-TIME PCRQ2

Abstract

Background: The increasing emergence of ANTIMICROBIAL RESISTANCE among gram negative bacteria especially Enterobacteriaceae has become a global problem. KLEBSIELLA PNEUMONIAE is an opportunistic pathogen, which recently due to causing broad spectrum of disease and antibiotic resistance has been lionized. EFFLUX PUMPS are one of the antibiotic resistance mechanisms, which were introduced in this bacteria. Therefore, the aims of this study were detection of oqxA, oqxB, acrA and acrB genes, evaluation of expression level of oqxA and acrA genes and determination of antibiotic resistance patterns among KLEBSIELLA PNEUMONIAE isolated at Tehran hospitals.Materials and Methods: In this descriptive study 83 KLEBSIELLA PNEUMONIAE were collected from patients attending to three hospitals in Tehran. Antibiotic susceptibility test was performed by disk diffusion method. The presence of oqxA, oqxB, acrA and acrB resistance genes was detected by PCR. The CCCP was used as efflux pump inhibitor and the oqxA, acrA genes expression level was analyzed by using REAL-TIME PCR.Results: The most of strains showed to have OqxAB (96%) and AcrAB (97%) pumps. The use of efflux pump inhibitor showed different results for ciprofloxacin MIC. REAL-TIME PCR assay showed higher expression level of OqxAB (2.3 folds) and AcrAB (4 folds) pumps in resistant strains.Conclusion: The existence of antibiotic resistance genes, increasing of KLEBSIELLA PNEUMONIAE strains, which become resistant to common antibiotics and nosocomial infections, highlights the need for particular detection, prevention and control of spread of resistant bacteria.

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APA: Copy

SAADATIAN FARIVAR, AREZOO, NOWROOZI, JAMILEH, ESLAMI, GITA, SABOKBAR, AZAR, & HASHEMI, ALI. (2016). THE STUDY OF ANTIBIOTIC RESISTANCE AMONG KLEBSIELLA PNEUMONIAE AND EXPRESSION LEVEL OF OQXA AND ACRA GENES BY USING REAL-TIME PCR. PAJOUHESH DAR PEZESHKI, 40(1 ), 42-48. SID. https://sid.ir/paper/41809/en

Vancouver: Copy

SAADATIAN FARIVAR AREZOO, NOWROOZI JAMILEH, ESLAMI GITA, SABOKBAR AZAR, HASHEMI ALI. THE STUDY OF ANTIBIOTIC RESISTANCE AMONG KLEBSIELLA PNEUMONIAE AND EXPRESSION LEVEL OF OQXA AND ACRA GENES BY USING REAL-TIME PCR. PAJOUHESH DAR PEZESHKI[Internet]. 2016;40(1 ):42-48. Available from: https://sid.ir/paper/41809/en

IEEE: Copy

AREZOO SAADATIAN FARIVAR, JAMILEH NOWROOZI, GITA ESLAMI, AZAR SABOKBAR, and ALI HASHEMI, “THE STUDY OF ANTIBIOTIC RESISTANCE AMONG KLEBSIELLA PNEUMONIAE AND EXPRESSION LEVEL OF OQXA AND ACRA GENES BY USING REAL-TIME PCR,” PAJOUHESH DAR PEZESHKI, vol. 40, no. 1 , pp. 42–48, 2016, [Online]. Available: https://sid.ir/paper/41809/en

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