INDENTIFICATION OF COMMONS PROTEINS BETWEEN EPIDIDYMAL EPITHELIUM SECRETIONS AND
SURFACE OF RAT SPERMATOZOA

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SADEGHI M.R.; AKHOUNDI M.A.; ZAHRAEI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Paper Information

Journal: Research in Medicine Year:2001 | Volume:24 | Issue:4 Page(s): 305-318

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Information Journal Paper

Title

INDENTIFICATION OF COMMONS PROTEINS BETWEEN EPIDIDYMAL EPITHELIUM SECRETIONS AND SURFACE OF RAT SPERMATOZOA

Author(s)

SADEGHI M.R. | AKHOUNDI M.A. | ZAHRAEI M. | Issue Writer Certificate

Keywords

EPIDIDYMISQ4

SPERMQ2

RATQ3

SPERM MATURATIONQ3

- SECRETORY PROTEINQ3

SECRETORYQ3

GLYCOPROTEINQ3

Abstract

Introduction: After SPERM leave the testis, further post testicular development is required in order fully functional SPERMatozoa capable of passing through the female tract and fertilizing ova. This process is called SPERM maturation and occurs in the EPIDIDYMIS, The epididymal epithelium provides the special microenvironment for SPERM maturation, these process involved remodeling. insertion, deletion or modification of many protein and lipid components of SPERM plasma membrane. Evaluation of such modification have a critical role in our understanding of epididymal function.Methods: Segments of washed caput and corpus regions of RAT EPIDIDYMIS were treated with enzymatic and mechanical digestion to removed connective tissue and produce fragments less than 1 mm length. Epididymal fragments were successfully cultured in modified RPMI -1640 medium supplemented with androgens and FCS.Pulse labelling of epididymal culture were performed in vitro with (35S) Met and (35S) Cys mixture. Protein synthesis and secretion were evaluated by SDS-PAGE and flurography. An antiserum against SPERM surface proteins was raised by repeated injections of RAT viable SPERMatozoa to a rabbit. The antiserum was checked by ELISA and Indirect Immunofluresence (IF) techniques. Identical proteins in epididymal secretions and SPERM surface were identified by western blotting. Results: Epididymal epithelia in culture displayed about 20 distinct protein bands. The most intensebands being at approximately 20-200 kD: A number of bands were evident less intensity. Immune serum reacted with 20, 24. 54, 58 kD protein bands after western blooting and were common between SPERM surface and epididymal epithelia secretion.Conclusion: Epithelial cells of EPIDIDYMIS secrete proteins and GLYCOPROTEINs, some of the binded to SPERM surface and probably have critical roles in SPERM-ooctyte interaction or membrance fusion. We have identified four proteins with molecular mass about 20, 24, 54, 58 kD that secreted by epididymal epithelia and bounded to SPERM surface. Our future research will focused on purification of the proteins and evaluation of their roles on fertilization process and SPERM fertilizing ability by IVF technique.

Cites

ROLE OF SPERM SURFACE PROTEINS WITH EPIDIDYMAL ORIGIN ON FERTILIZATION

References

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APA: Copy

SADEGHI, M.R., AKHOUNDI, M.A., & ZAHRAEI, M.. (2001). INDENTIFICATION OF COMMONS PROTEINS BETWEEN EPIDIDYMAL EPITHELIUM SECRETIONS AND SURFACE OF RAT SPERMATOZOA. PAJOUHESH DAR PEZESHKI, 24(4), 305-318. SID. https://sid.ir/paper/41867/en

Vancouver: Copy

SADEGHI M.R., AKHOUNDI M.A., ZAHRAEI M.. INDENTIFICATION OF COMMONS PROTEINS BETWEEN EPIDIDYMAL EPITHELIUM SECRETIONS AND SURFACE OF RAT SPERMATOZOA. PAJOUHESH DAR PEZESHKI[Internet]. 2001;24(4):305-318. Available from: https://sid.ir/paper/41867/en

IEEE: Copy

M.R. SADEGHI, M.A. AKHOUNDI, and M. ZAHRAEI, “INDENTIFICATION OF COMMONS PROTEINS BETWEEN EPIDIDYMAL EPITHELIUM SECRETIONS AND SURFACE OF RAT SPERMATOZOA,” PAJOUHESH DAR PEZESHKI, vol. 24, no. 4, pp. 305–318, 2001, [Online]. Available: https://sid.ir/paper/41867/en

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