CLONING OF COAGULANT VII FACTOR FROM HEPATOMA CELL LINE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHABANI PARISA; BANDEHPOOR MOJGAN; EMAMGHOLIPOOR SALALEH; MAGHEN LEILA; SEYED NEGAR; YAGHMAIE BAHRAM; KAZEMI BAHRAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Research in Medicine Year:2010 | Volume:34 | Issue:1 Page(s): 20-25

Download Full-Text

Persian Verion

View:

1,104

Download:

Cites:

Information Journal Paper

Title

CLONING OF COAGULANT VII FACTOR FROM HEPATOMA CELL LINE

Author(s)

Keywords

CLONINGQ3

COAGULANT VII FACTORQ3

HEPATOMA CELL LINEQ2

Abstract

Background: Factor VII, is a coagulant protease; it begins the proteolytic cascade reactions and produces thrombin. The use of recombinant human factor VII, (rhFVII) is effective for the treatment of patients with hemophilia A or B. It is a target for gene therapy. This study was done to clone factor VII from HepG2 cell line.Methods: RNA was extracted from the hepatoma, (HepG2), cell line. On reverse transcription FVII cDNA was amplified by RT-PCR. PCR product was cloned into the pTZ57R/T vector and transported into the E-coli cells.Results: By amplification of the FVII gene, the PCR band was observed and CLONING into the vector was confirmed by restriction analysis.Conclusion: In this paper we report the CLONING of factor VII from HepG2 cell line.

Cites

No record.

References

No record.

Cite

APA: Copy

SHABANI, PARISA, BANDEHPOOR, MOJGAN, EMAMGHOLIPOOR, SALALEH, MAGHEN, LEILA, SEYED, NEGAR, YAGHMAIE, BAHRAM, & KAZEMI, BAHRAM. (2010). CLONING OF COAGULANT VII FACTOR FROM HEPATOMA CELL LINE. PAJOUHESH DAR PEZESHKI, 34(1), 20-25. SID. https://sid.ir/paper/41900/en

Vancouver: Copy

SHABANI PARISA, BANDEHPOOR MOJGAN, EMAMGHOLIPOOR SALALEH, MAGHEN LEILA, SEYED NEGAR, YAGHMAIE BAHRAM, KAZEMI BAHRAM. CLONING OF COAGULANT VII FACTOR FROM HEPATOMA CELL LINE. PAJOUHESH DAR PEZESHKI[Internet]. 2010;34(1):20-25. Available from: https://sid.ir/paper/41900/en

IEEE: Copy

PARISA SHABANI, MOJGAN BANDEHPOOR, SALALEH EMAMGHOLIPOOR, LEILA MAGHEN, NEGAR SEYED, BAHRAM YAGHMAIE, and BAHRAM KAZEMI, “CLONING OF COAGULANT VII FACTOR FROM HEPATOMA CELL LINE,” PAJOUHESH DAR PEZESHKI, vol. 34, no. 1, pp. 20–25, 2010, [Online]. Available: https://sid.ir/paper/41900/en

Related Journal Papers