EFFECT OF AMNIOTIC FLUID ON THE INVITRO CULTURE OF HUMAN CORNEAL ENDOTHELIAL CELLS

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FEIZI S.; SOHEILI Z.S.; BAGHERI A.; BALAGHOLI S.; KARIMI AVAL S.

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Journal: BINA Year:2014 | Volume:19 | Issue:3 (76) Page(s): 222-233

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Title

EFFECT OF AMNIOTIC FLUID ON THE INVITRO CULTURE OF HUMAN CORNEAL ENDOTHELIAL CELLS

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Keywords

HUMAN CORNEAL ENDOTHELIAL CELLSQ3

HUMAN AMNIOTIC FLUIDQ3

INVITRO CULTUREQ3

Abstract

Purpose: To evaluate the effect of HUMAN AMNIOTIC FLUID (HAF) on the growth of HUMAN CORNEAL ENDOTHELIAL CELLS (HCECs) and to establish an in vitro method for the expansion of HCECs.Methods: HCECs were cultured in DMEM-F12 supplemented with 20% fetal bovine serum (FBS). Confluent monolayer cultures were trypsinized and passaged using 20% FBS- or 10%, 20%, or 30% HAF-containing media. Cell proliferation and cell death ELISA assays were performed to assess the effect of HAF on cell growth and viability. The identity of cells cultured with 20% HAF were studied using immunocytochemistry (ICC) and Real-Time RT-PCR techniques for markers presenting specific characteristics of HCECs including Ki-67, Vimentin, Na/K-ATPase and ZO-1.Results: Primary cultures of HCECs were successfully established under HAF-containing media which led to rapid cell proliferation according to cell proliferation and death ELISA assays. Real-Time PCR and ICC revealed that expression of Na/K-ATPase and ZO-1 in 20% HAF cell cultures was more compared to controls (20% FBS).Conclusion: HAF containing medium exhibited an invaluable stimulatory effect on HCEC growth. It can be regarded as an enriched supplement in HCECs regeneration studies.

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APA: Copy

FEIZI, S., SOHEILI, Z.S., BAGHERI, A., BALAGHOLI, S., & KARIMI AVAL, S.. (2014). EFFECT OF AMNIOTIC FLUID ON THE INVITRO CULTURE OF HUMAN CORNEAL ENDOTHELIAL CELLS. BINA, 19(3 (76)), 222-233. SID. https://sid.ir/paper/42295/en

Vancouver: Copy

FEIZI S., SOHEILI Z.S., BAGHERI A., BALAGHOLI S., KARIMI AVAL S.. EFFECT OF AMNIOTIC FLUID ON THE INVITRO CULTURE OF HUMAN CORNEAL ENDOTHELIAL CELLS. BINA[Internet]. 2014;19(3 (76)):222-233. Available from: https://sid.ir/paper/42295/en

IEEE: Copy

S. FEIZI, Z.S. SOHEILI, A. BAGHERI, S. BALAGHOLI, and S. KARIMI AVAL, “EFFECT OF AMNIOTIC FLUID ON THE INVITRO CULTURE OF HUMAN CORNEAL ENDOTHELIAL CELLS,” BINA, vol. 19, no. 3 (76), pp. 222–233, 2014, [Online]. Available: https://sid.ir/paper/42295/en

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