CULTURE AND PROLIFERATION OF HUMAN LIMBAL STEM CELLS IN VITRO

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BAHARVAND H.; EBRAHIMI M.; JAVADI M.A.; EYN ELAHI BAHRAM; MASOUMI M.

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Journal: BINA Year:2005 | Volume:10 | Issue:4 (40) Page(s): 419-429

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Information Journal Paper

Title

CULTURE AND PROLIFERATION OF HUMAN LIMBAL STEM CELLS IN VITRO

Author(s)

Keywords

LIMBALSTEM CELLSQ3

CORNEAL MARKERSQ3

AMNIOTIC MEMBRANEQ3

Abstract

Purpose: To evaluate the properties of cultured limbal stem cells for corneal surface reconstruction.Methods: Specimens of limbal explants were prepared from the Eye Bank of I.R. Iran. The explants were cultured and expanded on acellular AMNIOTIC MEMBRANE for 21 days. The cultured cells \vcrc evaluated for expression of Connexin 43 and keratinine K3 by immunocytochemistry and expression ofK 12 keratinine and P 63 by RT-PCR.Results: After 2 to 3 weeks, the limbal epithelial cells grew to form a sheet approximately 2×2 cm2 in size on the AMNIOTIC MEMBRANE. On histological examination the epithelial sheet was composed of 4 to 5 cell layers at the margin of the sheet and from 1 to 4 cells layers in the area betwecn the margin and the original explant tissue. Immunocytochemistry analysis showed Keratinine K3 and Connexin 43 were expressed in normal cornea. The markers were expressed wcakly in cultured limbal cells. Also, RT-PCR analysis revealed that P63 and K12 were expressed in cultured and normallimbal cells. The normal cornea expressed K3 and K12 but not P 63. Conclusions: These data support the notion that expansion of limbal stem cells on AMNIOTIC MEMBRANE can be used of tranplantation to patients with limbal stem cell deficiency.

Cites

EARLY RESULTS OF AUTOLOGOUS CULTIVATED LIMBAL STEM CELL TRANSPLANTATION IN TOTAL LIMBAL STEM CELL DEFICIENCY

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APA: Copy

BAHARVAND, H., EBRAHIMI, M., JAVADI, M.A., EYN ELAHI, BAHRAM, & MASOUMI, M.. (2005). CULTURE AND PROLIFERATION OF HUMAN LIMBAL STEM CELLS IN VITRO. BINA, 10(4 (40)), 419-429. SID. https://sid.ir/paper/42467/en

Vancouver: Copy

BAHARVAND H., EBRAHIMI M., JAVADI M.A., EYN ELAHI BAHRAM, MASOUMI M.. CULTURE AND PROLIFERATION OF HUMAN LIMBAL STEM CELLS IN VITRO. BINA[Internet]. 2005;10(4 (40)):419-429. Available from: https://sid.ir/paper/42467/en

IEEE: Copy

H. BAHARVAND, M. EBRAHIMI, M.A. JAVADI, BAHRAM EYN ELAHI, and M. MASOUMI, “CULTURE AND PROLIFERATION OF HUMAN LIMBAL STEM CELLS IN VITRO,” BINA, vol. 10, no. 4 (40), pp. 419–429, 2005, [Online]. Available: https://sid.ir/paper/42467/en

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