MORPHOLOGICAL AND MOLECULAR (28S RDNA) CHARACTERIZATION OF DICROCOELIUM DENDRITICUM ISOLATES FROM SHEEP, GOAT AND CATTLE IN MAZANDARAN PROVINCE, IRAN

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BARI SIMIN; ARBABI MOHSEN; GILL POORIA; SHARIF MEHDI; Ziaei Hezarjaribi Hajar; DODANGEH SAMIRA; Alizadeh Abbas; HEDAYATI ZEINAB; SARVI SHAHABEDDIN

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2018 | Volume:28 | Issue:159 Page(s): 38-45

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Title

MORPHOLOGICAL AND MOLECULAR (28S RDNA) CHARACTERIZATION OF DICROCOELIUM DENDRITICUM ISOLATES FROM SHEEP, GOAT AND CATTLE IN MAZANDARAN PROVINCE, IRAN

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Keywords

DICROCOELIUMQ2

MORPHOLOGYQ2

28S RDNAQ1

PCR-RFLPQ2

Abstract

Background and purpose: Dicrocoeliasis is a prevalent parasite in ruminants caused bydifferent species of DICROCOELIUM spp. This parasite lives in bile duct and gallbladder of ruminants andaccidentally in human. The economic significance of dicroceliasis is due to direct damage to the liver and, consequently, to the loss of significant amounts of protein. The present study aimed to investigate themorphometric and molecular (PCR and PCR-RFLP) parameters of DICROCOELIUM species isolates fromsheep, goat, and cattle in Mazandaran province, Iran during 2013-2015. Materials and methods: In this descriptive study, 50 trematodes isolated from livers of sheep(n= 20), goat (n= 20), and cattle (n= 10) were collected form abattoirs in Mazandaran province. Adultworms were studied by morphometric and molecular methods on 28S RDNA. The RFLP technique wasused to identify the genus. Some samples were also sequenced. Results: All isolates of three hosts (sheep, goat, and cattle) showed significant differences inmany morphological parameters. According to characterization of testicles that is a remarkablecharacteristic for differential diagnosis of DICROCOELIUM species, they were in tandem position in allisolates. Moreover, no significant difference was observed in their size in different hosts. Four bands, including 116, 145, 393, and 409 bp were produced from PCR method after treatment by enzyme. Inmolecular technique, there were no any significant differences but in morphometric analysis, somedifferences were found. Conclusion: The molecular and morphometric results indicated that D. dendriticum is the onlyspecies infecting sheep, goat, and cattle in Mazandaran province.

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APA: Copy

BARI, SIMIN, ARBABI, MOHSEN, GILL, POORIA, SHARIF, MEHDI, Ziaei Hezarjaribi, Hajar, DODANGEH, SAMIRA, Alizadeh, Abbas, HEDAYATI, ZEINAB, & SARVI, SHAHABEDDIN. (2018). MORPHOLOGICAL AND MOLECULAR (28S RDNA) CHARACTERIZATION OF DICROCOELIUM DENDRITICUM ISOLATES FROM SHEEP, GOAT AND CATTLE IN MAZANDARAN PROVINCE, IRAN. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 28(159 ), 38-45. SID. https://sid.ir/paper/44150/en

Vancouver: Copy

BARI SIMIN, ARBABI MOHSEN, GILL POORIA, SHARIF MEHDI, Ziaei Hezarjaribi Hajar, DODANGEH SAMIRA, Alizadeh Abbas, HEDAYATI ZEINAB, SARVI SHAHABEDDIN. MORPHOLOGICAL AND MOLECULAR (28S RDNA) CHARACTERIZATION OF DICROCOELIUM DENDRITICUM ISOLATES FROM SHEEP, GOAT AND CATTLE IN MAZANDARAN PROVINCE, IRAN. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;28(159 ):38-45. Available from: https://sid.ir/paper/44150/en

IEEE: Copy

SIMIN BARI, MOHSEN ARBABI, POORIA GILL, MEHDI SHARIF, Hajar Ziaei Hezarjaribi, SAMIRA DODANGEH, Abbas Alizadeh, ZEINAB HEDAYATI, and SHAHABEDDIN SARVI, “MORPHOLOGICAL AND MOLECULAR (28S RDNA) CHARACTERIZATION OF DICROCOELIUM DENDRITICUM ISOLATES FROM SHEEP, GOAT AND CATTLE IN MAZANDARAN PROVINCE, IRAN,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 28, no. 159 , pp. 38–45, 2018, [Online]. Available: https://sid.ir/paper/44150/en

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