EFFECTS OF HYDROALCOHOLIC EXTRACT OF SAPONARIA OFFICINALIS LEAF ON GROWTH OF TRICHOMONAS VAGINALIS IN VITRO

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Ziaei Hezarjaribi Hajar; Momeni Zohreh; AZADBAKHT MOHAMMAD; RAHIMI ESBOEI BAHMAN; FAKHAR MAHDI; AKBARIAN MESHKAT

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2016 | Volume:25 | Issue:134 Page(s): 52-59

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Title

EFFECTS OF HYDROALCOHOLIC EXTRACT OF SAPONARIA OFFICINALIS LEAF ON GROWTH OF TRICHOMONAS VAGINALIS IN VITRO

Author(s)

Keywords

SAPONARIA OFFICINALISQ2

LEAF EXTRACTQ2

IN VITROQ2

TRICHOMONAS VAGINALISQ2

Abstract

Background and purpose: SAPONARIA OFFICINALIS is a plant from the caryophyllaceae family and there are some contradictory reports about its antimicrobial effects. Metronidazole is commonly used in treatment of trichomoniasis but it has several side effects. On the other hand, finding an alternative drug from natural sources is very important. This study aimed at evaluating the hydroalcoholic extract of SAPONARIA OFFICINALIS leaf plant on the growth of TRICHOMONAS VAGINALIS IN VITRO.Materials and methods: The plant was approved in herbarium and hydro-alcoholic extracts were prepared. The experiment was done using 24 wells cell culture plate. In each well, 200 microliter of TYM culture medium and 200 microliter of different concentrations of plant extract was added. Also metronidazole was added to positive control well. Then 100 ml TYM were added to all wells containing 500,000 parasites and the plates were incubated at 37oC. The experiment was performed as double blind and triplicate. The mean number of the parasites in different concentrations and times and effect of the extract were recorded.Results: The number of parasites in 50, 100, 200, 400, 800 and 1600 micrograms per ml of the extract, during 24, 48, and 72 hr increased compared to the negative controls, while no growth of parasite was observed in positive controls (containing Metronidazole).Conclusion: SAPONARIA OFFICINALIS LEAF EXTRACT caused growth stimulation and increased the number of TRICHOMONAS VAGINALIS, therefore, it could be used for mass cultivation of the parasite IN VITRO. However, further investigations are needed in future.

Cites

IN VITRO EVALUATION OF HYDROALCOHOLIC LEAF EXTRACT OF CHENOPODIUM ALBUM ON GROWTH OF TRICHOMONASE VAGINALIS

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APA: Copy

Ziaei Hezarjaribi, Hajar, Momeni, Zohreh, AZADBAKHT, MOHAMMAD, RAHIMI ESBOEI, BAHMAN, FAKHAR, MAHDI, & AKBARIAN, MESHKAT. (2016). EFFECTS OF HYDROALCOHOLIC EXTRACT OF SAPONARIA OFFICINALIS LEAF ON GROWTH OF TRICHOMONAS VAGINALIS IN VITRO. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 25(134), 52-59. SID. https://sid.ir/paper/44347/en

Vancouver: Copy

Ziaei Hezarjaribi Hajar, Momeni Zohreh, AZADBAKHT MOHAMMAD, RAHIMI ESBOEI BAHMAN, FAKHAR MAHDI, AKBARIAN MESHKAT. EFFECTS OF HYDROALCOHOLIC EXTRACT OF SAPONARIA OFFICINALIS LEAF ON GROWTH OF TRICHOMONAS VAGINALIS IN VITRO. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;25(134):52-59. Available from: https://sid.ir/paper/44347/en

IEEE: Copy

Hajar Ziaei Hezarjaribi, Zohreh Momeni, MOHAMMAD AZADBAKHT, BAHMAN RAHIMI ESBOEI, MAHDI FAKHAR, and MESHKAT AKBARIAN, “EFFECTS OF HYDROALCOHOLIC EXTRACT OF SAPONARIA OFFICINALIS LEAF ON GROWTH OF TRICHOMONAS VAGINALIS IN VITRO,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 25, no. 134, pp. 52–59, 2016, [Online]. Available: https://sid.ir/paper/44347/en

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