PREPARATION OF CHITIN AND CHITOSAN FROM SHRIMP SHELLS AND DETERMINATION OF ITS DEGREE OF DEACETYLATION

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POURMORAD F.; EBRAHIMIZADEH M.A.; HONARI S.; EBRAHIMI P.; ORANGIAN M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2006 | Volume:16 | Issue:50 Page(s): 27-34

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Information Journal Paper

Title

PREPARATION OF CHITIN AND CHITOSAN FROM SHRIMP SHELLS AND DETERMINATION OF ITS DEGREE OF DEACETYLATION

Author(s)

Keywords

CHITOSANQ2

CHITINQ1

SHRIMP SHELLQ4

DEACETYLATION VALUEQ4

Abstract

Background and purpose: CHITIN, a natural polysaccharide, which is widely existed in the Arthropod shell such as crab, clobster, shrimp and fungi. CHITIN and its derivative CHITOSAN, a mucopolysaccharide like glycoaminoglycan have been reported to be useful for biomedical applications like wound healing and dressings, drug delivery agents, anti- cholesterolemic agents, blood anticoagulants, anti- tumor agents and immunoadjuvants. It is predicted that CHITOSAN will be one of the most demanding material in 2005. About 75% of this key material will be used in biomedical areas. Materials and methods: In this study CHITIN and CHITOSAN (deacetylated derivative) were prepared from SHRIMP SHELLs of Iran. SHRIMP SHELL powder was treated by an appropriate acid in a proper condition. After filtration the precipitated impure CHITIN was washed and purified. The precipitate was treated with NaOH and CHITOSAN was obtained in this stage. The product was mixed with hot KOH. CHITOSAN was purified after filtering and washing with acid and alkaline treatment. Results: CHITOSAN was prepared in a good yield from SHRIMP SHELLs and it was confirmed by analytical methods such as IR, NMR and electrophoresis. DEACETYLATION VALUE of the solvable CHITOSAN was calculated by using of IR and NMR. Conclusion: Massive production of CHITOSAN in pharmaceutical grades is possible with these techniques.

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