CANTHARIDIN-INDUCED APOPTOSIS IN LEISHMANIA MAJOR PROMASTIGOTES AND MACROPHAGES INFECTED BY LEISHMANIA MAJOR AMASTIGOTES IN VITRO

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MAROUFI YAHYA; GHAFFARIFAR FATEMEH; DALIMI ABD ALHOSEIN; SHARIFI ZOHREH; HASSAN ZUHAIR

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2012 | Volume:22 | Issue:89 Page(s): 31-40

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Title

CANTHARIDIN-INDUCED APOPTOSIS IN LEISHMANIA MAJOR PROMASTIGOTES AND MACROPHAGES INFECTED BY LEISHMANIA MAJOR AMASTIGOTES IN VITRO

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Keywords

APOPTOSISQ1

CANTHARIDINQ3

FLOW CYTOMETRYQ2

LEISHMANIA MAJORQ2

Abstract

Background and purpose: Leishmania is flagellated protozoa and causative agent of leishmaniosis. It is one of the main public health problems in developing countries. CANTHARIDIN is terpenoid component that exists in Meloidae and Oedemeridae beetles. Cantharid in is vesicant and could induce APOPTOSIS in cancerous cells. This study was performed to determine the role of cantharid in in inducing APOPTOSIS in the Leishmania promastigotes and macrophages infected with parasite.Materials and methods: In this experimental study the effect of cantharid in was evaluated at concentration range of 0.5-50 mg/mL on the L. major promastigotes and concentration range of 5, 20 and 50 mg/mL on macrophages infected with L. major after 24, 48 and 72 hrs by FLOW CYTOMETRY assay.Results: Results showed that cantharid in with concentration levels of 50 mg/mL and 0.5 mg/mL has 68.50% cytotoxicity (as 63.23% APOPTOSIS, 5.27% late APOPTOSIS and 0% necrosis) and 14.29% cytotoxicity (as 13.12% APOPTOSIS, 1.12% late APOPTOSIS and 0.05% necrosis) in promastigotes after 72hrs, respectively. Cytotoxicity 0.5 mg/mL and 50 mg/mL cantharid in in infected macrophages after 48hrs was 61.81% (as 43.42% APOPTOSIS, 1.27% late APOPTOSIS and 17.11% necrosis) and 44.44% (as 31.05% APOPTOSIS, 10.08% necrosis and 3.31% late APOPTOSIS), respectively. Cytotoxicity of 50 mg/mL and 5 mg/mL CANTHARIDIN in non-infected macrophages after 48hrs was also 49.34% (as 21.35% APOPTOSIS, 4.23% late APOPTOSIS and 23.76% necrosis) and 43.79% (as 34.90% APOPTOSIS, 7.27% necrosis and 1.61% late APOPTOSIS), respectively.Conclusion: This research indicates that cantharid in at different concentration and times could induce APOPTOSIS in L. major promastigotes and macrophages infected with amastigotes.

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APA: Copy

MAROUFI, YAHYA, GHAFFARIFAR, FATEMEH, DALIMI, ABD ALHOSEIN, SHARIFI, ZOHREH, & HASSAN, ZUHAIR. (2012). CANTHARIDIN-INDUCED APOPTOSIS IN LEISHMANIA MAJOR PROMASTIGOTES AND MACROPHAGES INFECTED BY LEISHMANIA MAJOR AMASTIGOTES IN VITRO. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 22(89), 31-40. SID. https://sid.ir/paper/44701/en

Vancouver: Copy

MAROUFI YAHYA, GHAFFARIFAR FATEMEH, DALIMI ABD ALHOSEIN, SHARIFI ZOHREH, HASSAN ZUHAIR. CANTHARIDIN-INDUCED APOPTOSIS IN LEISHMANIA MAJOR PROMASTIGOTES AND MACROPHAGES INFECTED BY LEISHMANIA MAJOR AMASTIGOTES IN VITRO. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2012;22(89):31-40. Available from: https://sid.ir/paper/44701/en

IEEE: Copy

YAHYA MAROUFI, FATEMEH GHAFFARIFAR, ABD ALHOSEIN DALIMI, ZOHREH SHARIFI, and ZUHAIR HASSAN, “CANTHARIDIN-INDUCED APOPTOSIS IN LEISHMANIA MAJOR PROMASTIGOTES AND MACROPHAGES INFECTED BY LEISHMANIA MAJOR AMASTIGOTES IN VITRO,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 22, no. 89, pp. 31–40, 2012, [Online]. Available: https://sid.ir/paper/44701/en

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