COMPARISON THE EFFECTS OF TNF-α, MCM AND POLY I-C IN MATURATION OF DENDRITIC CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

DELIREZH NOWRUZ; ASADI BEHNAZ

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2012 | Volume:21 | Issue:SUPPLEMENT 1 Page(s): 51-65

Download Full-Text

Persian Verion

View:

693

Download:

Cites:

Information Journal Paper

Title

COMPARISON THE EFFECTS OF TNF-α, MCM AND POLY I-C IN MATURATION OF DENDRITIC CELLS

Author(s)

DELIREZH NOWRUZ | ASADI BEHNAZ | Issue Writer Certificate

Keywords

DENDRITIC CELLQ2

MONOCYTE CONDITIONED MEDIUM (MCM)Q2

TNF-αQ2

POLY (I-C)Q2

Abstract

Background and purpose: Several laboratories have developed culture systems including maturation factors for human DC from peripheral blood monocytes. We comprehensively studied the effect of addition of poly (I-C) to standard maturation stimulus, MCM and TNF-a on maturation of monocyte derived DCs and their ability to elicit T cell responses.Materials and methods: A short (4-day) priming of plastic adherent monocytes with granulocytemacrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) followed by allogenic tumor antigen pulsing and addition of MCM and TNF-a with or without poly (I-C) to generate fully mature and stable DCs. Phenotypic and functional analysis were carried out using anti CD14, HLA-DR and CD83 monoclonal antibodies, mixed lymphocyte reaction (MLR), phagocytic activity and cytokine release by DC stimulated T lymphocytes.Results: We found that fully matured DCs with large amount cytoplasm and copious dendritic projections were visible at the end of culturing period in the presence of MCM and TNF-a with or without rpoly (I-C). Flow cytometric analysis using anti-CD14, HLA-DR and CD83 revealed that addition of poly (I-C) to conventional maturation factors results in decreased expression of CD14 and increased expression of HLA-DR and CD83. Functionally, MCM and TNF-a with poly (I-C) treated DCs showed a little stronger mixed leukocyte reaction Analysis of phagocytic activity showed that addition of poly (I-C) reduced FITC-conjugated bead uptake and increased mean fluorescent intensity (MFI) of phagocytic DCs. Furthermore our results revealed that additional treatment of DCs with poly (I-C) results in reduction of IL-12: IL-10 and IFN-γ: IL-4 ratios in DC and DC-primed T cell supernatants respectively.Conclusion: Our results support this idea that use of the MCM, TNF-a and poly (I-C) as maturation factor could gnerates more mature monocyte derived DCs that prime T lymphocytes to TH2 type cytokine release.

Cites

COMPARISON THE EFFECTS OF TWO MONOCYTE ISOLATION METHODS, PLASTIC ADHERENCE AND MAGNETIC ACTIVATED CELL SORTING METHODS, ON PHAGOCYTIC ACTIVITY OF GENERATED DENDRITIC CELLS

References

No record.

Cite

APA: Copy

DELIREZH, NOWRUZ, & ASADI, BEHNAZ. (2012). COMPARISON THE EFFECTS OF TNF-α, MCM AND POLY I-C IN MATURATION OF DENDRITIC CELLS. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 21(SUPPLEMENT 1), 51-65. SID. https://sid.ir/paper/44736/en

Vancouver: Copy

DELIREZH NOWRUZ, ASADI BEHNAZ. COMPARISON THE EFFECTS OF TNF-α, MCM AND POLY I-C IN MATURATION OF DENDRITIC CELLS. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2012;21(SUPPLEMENT 1):51-65. Available from: https://sid.ir/paper/44736/en

IEEE: Copy

NOWRUZ DELIREZH, and BEHNAZ ASADI, “COMPARISON THE EFFECTS OF TNF-α, MCM AND POLY I-C IN MATURATION OF DENDRITIC CELLS,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 21, no. SUPPLEMENT 1, pp. 51–65, 2012, [Online]. Available: https://sid.ir/paper/44736/en

Related Journal Papers