CULTURING OF KERATIONOCYTES IN SERUM-FREE MEDIUM

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SHOKRZADEH M.; MAHMOUDIRAD M.; MIRZAEI MANSOUREH; MOSEF N.; AHANGARI M.

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Journal Paper

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2009 | Volume:19 | Issue:70 Page(s): 67-71

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Title

CULTURING OF KERATIONOCYTES IN SERUM-FREE MEDIUM

Author(s)

Keywords

KERATINOCYTEQ3

EPIDERMAL EXPLANTSQ2

SERUM-FREE MEDIUMQ3

Abstract

Background and purpose: To establish a simple method for preparation of pure epidermal cell in SERUM-FREE MEDIUM, without co culturing with lethally irradiated 3T3 cells.Materials and methods: A piece of skin biopsy was taken from a rat. After trypsinazation to separate epidermal layer, the epidermis was cut into explants 1×1 mm and were laid at 50 mL on tissue cultured flasks. Then, the explants were covered with serum-free KERATINOCYTE growth medium, while the culture medium was changed every two days. The KERATINOCYTE colonies were expanded in flasks and proliferated to 70% confluency on day 21. After two subcultures, the cells were frozen.Results: Pure epidermal cells are established by this technique in a serum-free KERATINOCYTE growth medium, under feeder cell-free condition.Conclusion: Culturing of KERATINOCYTEs in SERUM-FREE MEDIUM is proved to be a useful and simple method for KERATINOCYTE isolation. The successful culturing of KERATINOCYTEs from a small skin biopsy can be useful in the treatment of major burn wounds.

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APA: Copy

SHOKRZADEH, M., MAHMOUDIRAD, M., MIRZAEI, MANSOUREH, MOSEF, N., & AHANGARI, M.. (2009). CULTURING OF KERATIONOCYTES IN SERUM-FREE MEDIUM. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 19(70), 67-71. SID. https://sid.ir/paper/44943/en

Vancouver: Copy

SHOKRZADEH M., MAHMOUDIRAD M., MIRZAEI MANSOUREH, MOSEF N., AHANGARI M.. CULTURING OF KERATIONOCYTES IN SERUM-FREE MEDIUM. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2009;19(70):67-71. Available from: https://sid.ir/paper/44943/en

IEEE: Copy

M. SHOKRZADEH, M. MAHMOUDIRAD, MANSOUREH MIRZAEI, N. MOSEF, and M. AHANGARI, “CULTURING OF KERATIONOCYTES IN SERUM-FREE MEDIUM,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 19, no. 70, pp. 67–71, 2009, [Online]. Available: https://sid.ir/paper/44943/en

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