DIFFERENTIATION OF HUMAN WHARTON'S JELLY MESENCHYMAL STEM CELLS INTO OOCYTE-LIKE CELLS BY FOLLICULAR FLUID

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ZOLFAGHAR MONA; FATHI ROUHOLLAH; NAJI TAHEREH

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2017 | Volume:27 | Issue:154 Page(s): 1-11

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Title

DIFFERENTIATION OF HUMAN WHARTON'S JELLY MESENCHYMAL STEM CELLS INTO OOCYTE-LIKE CELLS BY FOLLICULAR FLUID

Author(s)

ZOLFAGHAR MONA | FATHI ROUHOLLAH | NAJI TAHEREH | Issue Writer Certificate

Keywords

STEM CELLQ2

WHARTON’S JELLYQ1

FOLLICULAR FLUIDQ3

OOCYTE-LIKE CELLSQ1

Abstract

Background and purpose: FOLLICULAR FLUID (FF) is a rich source of compounds for the development of oocyte. Wharton's jelly mesenchymal STEM CELLs (WJ-MSCs) have a same differentiation origin with primordial germ cells, therefore, WJ-MSCs could differentiate into OOCYTE-LIKE CELLS (OLCs) in the presence of appropriate factors. The purpose of present study was to isolate WJ-MSCs and then investigate their differentiation capacity to OLCs by FF.Materials and methods: The fragments of Wharton's jelly were cultured in α-MEM supplemented with 10% FBS. WJ-MSC at the 3rd passage were studied for differentiation ability to adipocytes and osteocytes. Furthermore, WJ-MSCs related markers were assessed by flow cytometry. In the same passage, WJ-MSCs were induced to differentiate into OOCYTE-LIKE CELLS by adding 10% human FF in α-MEM for 21 days.Results: WJ-MSCs could differentiate into adipocytes and osteocytes. Flow cytometry analysis indicated that WJ-MSCs do not express hematopoietic marker (CD34-CD45) and express MSCs markers (CD73, CD90 and CD105). WJ-MSCs which were under influence of FF differentiated into OLCs and immunocytochemistry analysis showed these cells have positive expression of ZP3, SYCP3 (oocyte’s markers) and VASA (germ cell’s marker).Conclusion: The present study demonstrated that WJ-MSCs could differentiate into OLCs (morphologically) under the influence of FF and also expressed ZP3, VASA, and SYCP3 markers positively. According to the capabilities of WJ-MSCs, these cells seem to be suitable for use in cell therapy projects to improve infertility treatments.

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APA: Copy

ZOLFAGHAR, MONA, FATHI, ROUHOLLAH, & NAJI, TAHEREH. (2017). DIFFERENTIATION OF HUMAN WHARTON'S JELLY MESENCHYMAL STEM CELLS INTO OOCYTE-LIKE CELLS BY FOLLICULAR FLUID. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 27(154 ), 1-11. SID. https://sid.ir/paper/45159/en

Vancouver: Copy

ZOLFAGHAR MONA, FATHI ROUHOLLAH, NAJI TAHEREH. DIFFERENTIATION OF HUMAN WHARTON'S JELLY MESENCHYMAL STEM CELLS INTO OOCYTE-LIKE CELLS BY FOLLICULAR FLUID. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;27(154 ):1-11. Available from: https://sid.ir/paper/45159/en

IEEE: Copy

MONA ZOLFAGHAR, ROUHOLLAH FATHI, and TAHEREH NAJI, “DIFFERENTIATION OF HUMAN WHARTON'S JELLY MESENCHYMAL STEM CELLS INTO OOCYTE-LIKE CELLS BY FOLLICULAR FLUID,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 27, no. 154 , pp. 1–11, 2017, [Online]. Available: https://sid.ir/paper/45159/en

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