RAPID DETECTION OF FUNGAL PATHOGENS IN BLOOD CULTURES OF SEVERELY BURNED PATIENTS USING PANFUNGAL PCR ASSAY

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ABDOLLAHI AKRAM; SHOKOHI TAHEREH; HEDAYATI MOHAMMAD TAGHI; NABILI MOJTABA; LOTFI NAZANIN

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2016 | Volume:25 | Issue:132 Page(s): 219-228

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Title

RAPID DETECTION OF FUNGAL PATHOGENS IN BLOOD CULTURES OF SEVERELY BURNED PATIENTS USING PANFUNGAL PCR ASSAY

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Keywords

INVASIVE FUNGAL DISEASEQ2

BURNQ2

FUNGEMIAQ2

CANDIDAQ2

ASPERGILLUSQ2

Abstract

Background and purpose: FUNGEMIA is a significant cause of morbidity and mortality among immunocompromised patients such as severe BURNs. Due to low sensitivity and long turnaround time of the traditional biphasic BHI blood culture to detect FUNGEMIA we aimed to detect fungal elements in blood culture of these patients suspected with INVASIVE FUNGAL DISEASE (IFDs) using panfungal PCR assay.Materials and methods: Four hundred blood cultures were obtained from 112 severely BURNed patients suspected with IFDs. DNA was extracted and a pair of fungal universal primers was used to amplify the 18SrRNA gene. The PCR product was sequenced and identified using the nucleotide Basic Local Alignment Search Tool (BLAST).Results: 44(39.2%) Blood culture of 112 patients (mean age: 31.9±14.7 years) were positive. In PCR positive patients, mean BURN size was 42.6±19 % of total body surface area and average hospital length of stay was 21.8 ±10.3 days. In three patients, same species of fungi were determined in both sequencing of PCR products and the classical procedures of blood culture. Randomly sequencing of 20 PCR products revealed ASPERGILLUS fumigatus (n=11 55%), CANDIDA tropicalis (n=5 25%), C. albicans (n=1 5%), C. parapsilosis (n=1 5%), Agaricomycotina (n=1 5%), and Penicillium (n=1 5%) as causative agents.Conclusion: Panfungal PCR assay on blood culture seems to be a promising method for rapid detection of fungi in blood culture of patients at risk for IFDs.

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APA: Copy

ABDOLLAHI, AKRAM, SHOKOHI, TAHEREH, HEDAYATI, MOHAMMAD TAGHI, NABILI, MOJTABA, & LOTFI, NAZANIN. (2016). RAPID DETECTION OF FUNGAL PATHOGENS IN BLOOD CULTURES OF SEVERELY BURNED PATIENTS USING PANFUNGAL PCR ASSAY. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 25(132), 219-228. SID. https://sid.ir/paper/45287/en

Vancouver: Copy

ABDOLLAHI AKRAM, SHOKOHI TAHEREH, HEDAYATI MOHAMMAD TAGHI, NABILI MOJTABA, LOTFI NAZANIN. RAPID DETECTION OF FUNGAL PATHOGENS IN BLOOD CULTURES OF SEVERELY BURNED PATIENTS USING PANFUNGAL PCR ASSAY. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;25(132):219-228. Available from: https://sid.ir/paper/45287/en

IEEE: Copy

AKRAM ABDOLLAHI, TAHEREH SHOKOHI, MOHAMMAD TAGHI HEDAYATI, MOJTABA NABILI, and NAZANIN LOTFI, “RAPID DETECTION OF FUNGAL PATHOGENS IN BLOOD CULTURES OF SEVERELY BURNED PATIENTS USING PANFUNGAL PCR ASSAY,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 25, no. 132, pp. 219–228, 2016, [Online]. Available: https://sid.ir/paper/45287/en

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