IMMUNOGENICITY OF CHITOSAN NANOGEL CONTAINING IPAC RECOMBINANT PROTEIN FROM SHIGELLA IN GUINEA PIG

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NAZARIAN SHAHRAM; MOUSAVI GARGARI SEYED LATIF; AZIMBEYK MEHDI

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2017 | Volume:27 | Issue:149 Page(s): 14-27

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

737

Download:

Cites:

Information Journal Paper

Title

IMMUNOGENICITY OF CHITOSAN NANOGEL CONTAINING IPAC RECOMBINANT PROTEIN FROM SHIGELLA IN GUINEA PIG

Author(s)

NAZARIAN SHAHRAM | MOUSAVI GARGARI SEYED LATIF | AZIMBEYK MEHDI | Issue Writer Certificate

Keywords

NANOGELQ2

CHITOSANQ3

SHIGELLAQ1

IPACQ1

IMMUNOGENQ1

Abstract

Background and purpose: SHIGELLA species cause shigellosis in humans. Considering the high frequency of illness and antibiotic resistance, development of an effective IMMUNOGEN against bacteria is a major goal. Invasion Plasmid Antigen such as IpaD and IPAC are the major bacterial virulence agents of SHIGELLA. Encapsulation of antigens in particular carriers such as CHITOSANic NANOGELs, not only protects them from degradation in environmental elements but also provides the effective concentrations of antigens in targets, hence increasing bioactivity. The aim of this study was to investigate the IMMUNOGENic properties of IPAC protein encapsulated in CHITOSANic NANOGELs.Materials and methods: The protein was expressed in E.coli and purified by affinity chromatography. CHITOSAN NANOGELs were prepared by ionic gelation method using tripolyphosphate (TPP) as a crosslinking agent. The NANOGELs were loaded with IPAC and their structures were characterized by SEM and DLS. Encapsulated protein was introduced in guinea pigs by oral and parenteral routes. Antibody titers were determined by ELISA. Animals were challenged By Sereny test with wild-type S. flexneri.Results: Expression of recombinant protein in E.coli led to the production of IPAC with 60 kDa molecular weight. Loading efficiency of NANOGEL was 98% after 48h of incubation. The average particle size was 418 nm. Immunization of mice induced serum antibody response.Conclusion: The productivity of encapsulated protein via oral route was better than parenteral route.

Cites

No record.

References

Cite

APA: Copy

NAZARIAN, SHAHRAM, MOUSAVI GARGARI, SEYED LATIF, & AZIMBEYK, MEHDI. (2017). IMMUNOGENICITY OF CHITOSAN NANOGEL CONTAINING IPAC RECOMBINANT PROTEIN FROM SHIGELLA IN GUINEA PIG. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 27(149), 14-27. SID. https://sid.ir/paper/45460/en

Vancouver: Copy

NAZARIAN SHAHRAM, MOUSAVI GARGARI SEYED LATIF, AZIMBEYK MEHDI. IMMUNOGENICITY OF CHITOSAN NANOGEL CONTAINING IPAC RECOMBINANT PROTEIN FROM SHIGELLA IN GUINEA PIG. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;27(149):14-27. Available from: https://sid.ir/paper/45460/en

IEEE: Copy

SHAHRAM NAZARIAN, SEYED LATIF MOUSAVI GARGARI, and MEHDI AZIMBEYK, “IMMUNOGENICITY OF CHITOSAN NANOGEL CONTAINING IPAC RECOMBINANT PROTEIN FROM SHIGELLA IN GUINEA PIG,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 27, no. 149, pp. 14–27, 2017, [Online]. Available: https://sid.ir/paper/45460/en

Related Journal Papers