ECT OF GLUTATHION ON RESUMPTION OF MEIOSI IN-VITRO MATURATION, AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES

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IMANI H.; HASANI F.; HAERI ROUHANI SEYED ALI; NASRESFAHANI MOHAMMAD HOSSEIN; REZAZADEH M.; DALMAN A.; KAZEMI ASHTIANI S.; SHAHVERDI A.

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Journal: Journal of Mazandaran University of Medical Sciences Year:2005 | Volume:15 | Issue:48 Page(s): 1-10

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Title

ECT OF GLUTATHION ON RESUMPTION OF MEIOSI IN-VITRO MATURATION, AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES

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Keywords

GLUTATHIONQ2

IMMATURE OOCYTESQ2

MOUSEQ2

Abstract

Background and purpose: In this study the effects of GLUTATHION on resumption of meiosis, in-vitro maturation and development of immature MOUSE oocytes was evaluated. Materials and methods: IMMATURE OOCYTES recovered from NMRI MOUSE strain (4-6 weeks) in four different experimental groups. Group 1 (control group): 345 IMMATURE OOCYTES were matured in MEM-α medium containing FCS 5%, group 1: 338 IMMATURE OOCYTES were cultured in MEM-α medium containing FCS 5% and 1mM GLUTATHIONe, group 2: 237 IMMATURE OOCYTES curtured in MEM-α medium containing FCS 5%, 7.5 IuhCG, 100mIUrFSH and group 3: 327 IMMATURE OOCYTES were grew in MEM-α medium FCS5%, 7.5 IUhCG, 100mIUrFSH, 1mM GLUTATHIONe. Fertilization and embryo development were done in T6 medium. Results : There were significant differences between in-vitro maturation rates (group 1: 59.71%, group 2: 79.28%, group 3: 52.74 and group 4: 70.22%) and resumption of meiosis (group 1: 74.20%, group 2: 92.88%, group 3: 74.26%, group 4: 86.20%) in different groups. Conclusion: The results of this study showed that GLUTATHIONe (1mM) enhanced resumption of meiosis and in-vitro maturation and development of embryoes.

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APA: Copy

IMANI, H., HASANI, F., HAERI ROUHANI, SEYED ALI, NASRESFAHANI, MOHAMMAD HOSSEIN, REZAZADEH, M., DALMAN, A., KAZEMI ASHTIANI, S., & SHAHVERDI, A.. (2005). ECT OF GLUTATHION ON RESUMPTION OF MEIOSI IN-VITRO MATURATION, AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES . JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 15(48), 1-10. SID. https://sid.ir/paper/46040/en

Vancouver: Copy

IMANI H., HASANI F., HAERI ROUHANI SEYED ALI, NASRESFAHANI MOHAMMAD HOSSEIN, REZAZADEH M., DALMAN A., KAZEMI ASHTIANI S., SHAHVERDI A.. ECT OF GLUTATHION ON RESUMPTION OF MEIOSI IN-VITRO MATURATION, AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES . JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2005;15(48):1-10. Available from: https://sid.ir/paper/46040/en

IEEE: Copy

H. IMANI, F. HASANI, SEYED ALI HAERI ROUHANI, MOHAMMAD HOSSEIN NASRESFAHANI, M. REZAZADEH, A. DALMAN, S. KAZEMI ASHTIANI, and A. SHAHVERDI, “ECT OF GLUTATHION ON RESUMPTION OF MEIOSI IN-VITRO MATURATION, AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES ,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 15, no. 48, pp. 1–10, 2005, [Online]. Available: https://sid.ir/paper/46040/en

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